Abstract:
Objective To understand the antibiotic resistance, virulence genes and molecular characteristics of 36 Pseudomonas aeruginosa strains isolated from rural drinking water in Changping district, Beijing. Methods The strains were isolated and identified according to GB/T 8538-2008, and related antibiotic susceptibility test, virulence gene detection, and pulsed-field gel electrophoresis (PFGE) were conducted. Results Among the 36 P. aeruginosa strains isolated, 27 were isolated from finished water samples. All the strains were completely sensitive to 12 antibiotics, i.e. piperacillin, piperacillin/tazobactam, ceftazidime, cefepime, aztreonam, imipenem, meropenem, Amikacin, gentamicin, tobramycin, ciprofloxacin and levofloxacin. In 36 P. aeruginosa strains, 4 virulence gene profiles (T1-T4) were found, T1 (lasI/lasR/rhlI/rhlR/toxA/plcH/rhlAB/lasB) was the major gene profile, and 11 PFGE patterns were found, 0003 and 0010 were predominant. Conclusion P. aeruginosa strains, which were antibiotic sensitive, were mainly isolated from the finished water, 0003 and 0010 were the predominant PFGE patterns, and T1 was the predominant gene profile in Changping.