张婧, 李加全, 冯云, 韩茜, 潘虹, 杨和仙, 张海林, 宋志忠. 云南省保山市蚊虫中检测到基因Ⅰ型流行性乙型脑炎病毒[J]. 疾病监测, 2018, 33(2): 150-154. DOI: 10.3784/j.issn.1003-9961.2018.02.009
引用本文: 张婧, 李加全, 冯云, 韩茜, 潘虹, 杨和仙, 张海林, 宋志忠. 云南省保山市蚊虫中检测到基因Ⅰ型流行性乙型脑炎病毒[J]. 疾病监测, 2018, 33(2): 150-154. DOI: 10.3784/j.issn.1003-9961.2018.02.009
Zhang Jing, Li Jiaquan, Feng Yun, Han Xi, Pan Hong, Yang Hexian, Zhang Hailin, Song Zhizhong. Identification of Japanese encephalitis virus genotype Ⅰ from mosquitoes in Baoshan,Yunnan[J]. Disease Surveillance, 2018, 33(2): 150-154. DOI: 10.3784/j.issn.1003-9961.2018.02.009
Citation: Zhang Jing, Li Jiaquan, Feng Yun, Han Xi, Pan Hong, Yang Hexian, Zhang Hailin, Song Zhizhong. Identification of Japanese encephalitis virus genotype Ⅰ from mosquitoes in Baoshan,Yunnan[J]. Disease Surveillance, 2018, 33(2): 150-154. DOI: 10.3784/j.issn.1003-9961.2018.02.009

云南省保山市蚊虫中检测到基因Ⅰ型流行性乙型脑炎病毒

Identification of Japanese encephalitis virus genotype Ⅰ from mosquitoes in Baoshan,Yunnan

  • 摘要: 目的 调查云南省保山市蚊虫及其自然感染虫媒病毒状况,为蚊媒病毒病防控提供参考依据。方法 2015年9月采用诱蚊灯法在保山市隆阳区农村人房和畜圈采集蚊虫标本,反转录聚合酶链反应(RT-PCR)用于检测蚊虫标本中黄病毒属、甲病毒属、布尼亚病毒属和流行性乙型脑炎病毒(乙脑病毒)等相关病毒核酸,阳性者进行C/prM基因序列测定、同源性和系统进化分析。结果 共捕获蚊虫3种8 202只,三带喙库蚊(Culex tritaeniorhynchus)、中华按蚊(Anopheles sinensis)和骚扰阿蚊(Armigeres subalbatus)构成比依次为88.50%(7 259只)、11.43%(937只)和0.07%(6只)。蚊虫标本分30批进行RT-PCR检测,14批三带喙库蚊中检测到乙脑病毒核酸,并获得14株乙脑病毒C/prM基因核苷酸序列。同源性和进化分析表明,14株乙脑病毒与基因Ⅰ型乙脑病毒同在一个进化分支,并与2007年保山市腾冲县和2010年德宏傣族景颇族自治州(芒市和瑞丽市)的基因Ⅰ型乙脑病毒具有较近的进化关系。结论 三带喙库蚊为保山市的优势蚊种和乙脑病毒主要传播媒介,当地存在基因Ⅰ型乙脑病毒流行,此为近10年保山市首次检测到基因Ⅰ型乙脑病毒。

     

    Abstract: Objective To investigate the distribution of mosquitoes and their natural infection with mosquito-borne viruses in Baoshan of Yunnan province,and provide scientific evidence for the prevention and control of mosquito-borne diseases. Methods Mosquito samples were collected by using mosquito traps in the night from houses and livestock stables in villages in Baoshan in September 2015. The mosquito samples were used to detect the viral RNA with Reverse transcription-polymerase chain reaction (RT-PCR) using Flavivirus,Alphavirus,Bunyavirus and Japanese encephalitis virus (JEV) primers,and positive specimens were sequenced for C/prM gene for homology and phylogenetic analysis. Results A total of 8 202 mosquitoes in 3 species were collected in Longyang district of Baoshan. Culex tritaeriorhynchus, Anopheles sinensis and Armigeres subalbatus accounted for 88.50%(7 259),11.43%(937)and 0.07%(6)of the total,respectively. Thirty mosquito batches were detected by using RT-PCR,and JEV RNA were detected from 14 batches of Cx. tritaeriorhynchus. The sequences of C/prM genes of the 14 JEV strains were obtained. Homology and phylogenetic analysis showed that the 14 JEV strains were in the same clade of genotype Ⅰ(G-Ⅰ)strains of JEV from GenBank,indicating they belonged to G-Ⅰ, and all the JEV strains detected were closely related to the JEV G-Ⅰ strains from Tengchong county of Baoshan in 2007 and adjacent Mangshi and Ruili counties of Dehong in 2010. Conclusion Cx. tritaeriorhynchus was dominant mosquito species and the major transmitting vector of JEV in Baoshan. JEV G-Ⅰ was confirmed to circulate in the area. It was first time to detect JEV G-Ⅰ in Baoshan since 2007.

     

/

返回文章
返回