商晓春, 周晓红, 帅慧群, 赵雪琴, 张睿. 一起多血清型副溶血性弧菌引起的食物中毒脉冲场凝胶电泳分析[J]. 疾病监测, 2013, 28(7): 598-602. DOI: 10.3784/j.issn.1003-9961.2013.7.023
引用本文: 商晓春, 周晓红, 帅慧群, 赵雪琴, 张睿. 一起多血清型副溶血性弧菌引起的食物中毒脉冲场凝胶电泳分析[J]. 疾病监测, 2013, 28(7): 598-602. DOI: 10.3784/j.issn.1003-9961.2013.7.023
SHANG Xiao-chun, ZHOU Xiao-hong, SHUAI Hui-qun, ZHAO Xue-qin, ZHANG Rui. PFGE analysis of a food poisoning event caused by multi-serotype Vibrio parahemolyticus[J]. Disease Surveillance, 2013, 28(7): 598-602. DOI: 10.3784/j.issn.1003-9961.2013.7.023
Citation: SHANG Xiao-chun, ZHOU Xiao-hong, SHUAI Hui-qun, ZHAO Xue-qin, ZHANG Rui. PFGE analysis of a food poisoning event caused by multi-serotype Vibrio parahemolyticus[J]. Disease Surveillance, 2013, 28(7): 598-602. DOI: 10.3784/j.issn.1003-9961.2013.7.023

一起多血清型副溶血性弧菌引起的食物中毒脉冲场凝胶电泳分析

PFGE analysis of a food poisoning event caused by multi-serotype Vibrio parahemolyticus

  • 摘要: 目的 对一起食物中毒检出的3种血清型副溶血性弧菌(Vibrio parahaemolyticus,VP),采用脉冲场凝胶电泳(PFGE)进行同源性分析,以明确其传染源。 方法 参照 WS 271-2007等标准,进行副溶血性弧菌分离、生化和血清学鉴定、KP试验、药敏试验、毒力基因检测、PFGE分子分型,利用BioNumerics 软件对PFGE 分型图谱进行聚类分析。 结果 17份样品均检出副溶血性弧菌,血清型可分3个型别,以O3:K6为优势血清型,占70.59%,O2:K3和 O2:K28 两血清型所占比例不一。PFGE分子分型,按100%相似度可分6个基因型别,XC12001型为优势型别,占58.82%, XC12002~XC12006型所占比例不一,聚类分析显示XC12001和XC12002型、XC12004和XC12005型相似度较高。17株副溶血性弧菌药敏结果基本一致,对氨苄西林和阿莫西林耐药率高达100%。10株O3:K6型副溶血性弧菌只携带tdh毒力基因,其余菌株tdh、trh1、trh2毒力基因全部阴性。 结论 此起食物中毒是以O3:K6血清型为主的不同克隆群副溶血性弧菌混合感染所致,PFGE可有效应用于食物中毒溯源分析及分子流行病学研究。

     

    Abstract: Objective To understand the infection source of a food poisoning event by using pulsed-field gel electrophoresis (PFGE) to detect three serotypes of Vibrio parahemolyticus isolates to know their homology and virulence factors. Methods According to WS 271-2007 and other standards, the isolation, biochemical and serological identification, KP test, drug susceptibility test and virulence gene detection of Vibrio parahemolyticus strains were conducted and PFGE molecular typing was conducted. Results Vibrio parahemolyticus strains were isolated from 17 samples, which belonged to three serotypes. Serotype O3:K6 was predominant, accounting for 70.59%. The serotypes O2:K3 and O2:K28 had different proportions. Six genotypes were detected by PFGE molecular typing, based on 100% similarity. Genotype XC12001 was predominant, accounting for 58.82%, while genotypes XC12002-XC12006 had different proportions. Cluster analysis showed that XC12001 and XC12002,XC12004 and XC12005 had close genetic relationship. The susceptibility test results of 17 strains of Vibrio parahemolyticus were similar. The ampicillin and amoxicillin resistance rates of all strains were 100%. Ten strains of Vibrio parahemolyticus O3:K6 carried tdh virulence genes, while the test results of tdh, trh1 and trh2 gens of other Vibrio parahemolyticus strains were all negative. Conclusion This food poisoning event was caused by co-infections of different clonal Vibrio parahemolyticus, with serotype O3:K6 being predominant. PFGE can be used in the source tracing and molecular epidemiological study of food poisoning.

     

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