龙永艳, 倪贤生, 李端, 王瑞白, 陈海婴, 李娟, 阚飙. 食品从业人员肠道细菌CTX-M 型超广谱-内酰胺酶的携带状况研究[J]. 疾病监测, 2016, 31(5): 433-436. DOI: 10.3784/j.issn.1003-9961.2016.05.018
引用本文: 龙永艳, 倪贤生, 李端, 王瑞白, 陈海婴, 李娟, 阚飙. 食品从业人员肠道细菌CTX-M 型超广谱-内酰胺酶的携带状况研究[J]. 疾病监测, 2016, 31(5): 433-436. DOI: 10.3784/j.issn.1003-9961.2016.05.018
LONG Yong-yan, NI Xian-sheng, LI Duan, WANG Rui-bai, CHEN Hai-ying, LI Juan, KAN Biao. Carriage of CTX-M type extended-spectrum -lactamase in intestinal bacteria isolated from people engaged in food processing[J]. Disease Surveillance, 2016, 31(5): 433-436. DOI: 10.3784/j.issn.1003-9961.2016.05.018
Citation: LONG Yong-yan, NI Xian-sheng, LI Duan, WANG Rui-bai, CHEN Hai-ying, LI Juan, KAN Biao. Carriage of CTX-M type extended-spectrum -lactamase in intestinal bacteria isolated from people engaged in food processing[J]. Disease Surveillance, 2016, 31(5): 433-436. DOI: 10.3784/j.issn.1003-9961.2016.05.018

食品从业人员肠道细菌CTX-M 型超广谱-内酰胺酶的携带状况研究

Carriage of CTX-M type extended-spectrum -lactamase in intestinal bacteria isolated from people engaged in food processing

  • 摘要: 目的 调查食品从业人员肠道革兰阴性细菌CTX-M型超广谱-内酰胺酶(ESBLs)基因的携带状况及基因型分布。方法 选择140名食品从业人员健康体检时的肛拭子标本,用加入头孢曲松钠的营养琼脂平板筛选样本中革兰染色阴性耐药菌,PCR方法扩增blaCTX-M耐药基因,对阳性扩增产物进行测序以明确CTX基因亚型。结果 140份肛拭样本中有125份样本中分离到头孢曲松钠耐药革兰阴性菌,携带率为89.3%(125/140)。125株耐药菌株中CTX-M的检出率为97.6%;五组CTX中检出率最高的为CTX-M-9组56.0%,其次为CTX-M-1组38.4%,有4株菌同时检测到CTX-M-9组和CTX-M-1组酶。通过序列比对,共检测到9种CTX基因亚型,检出率最高的是CTX-M-14和CTX-M-15。结论 食品行业从业人员对-内酰胺类药物普遍存在有抗性的肠道细菌,产CTX-M型超广谱-内酰胺酶的革兰染色阴性细菌广泛存在,鉴于其工作特点,获得并导致耐药菌传播的风险性很高。

     

    Abstract: Objective To investigate the carriage and genotypes of CTX-M type extended-spectrum -lactamase (ESBLs) in the intestinal bacteria isolated from people engaged in food processing. Methods A total of 140 rectal swabs were collected from the people engaged in food processing. For each swab, ceftriaxone resistant bacteria were isolated using nutrient agar plateadding ceftriaxone sodium.blaCTX-M genes were detected by PCR for each strains. The genotypes of CTX-M were identified by sequencing of PCR positive amplification products. Results Ceftriaxoneresistant strains were successfully isolated from 125 of 140 stool samples (89.3%, 125/140). Among the 125 strains, 122(122/125, 97.6%) strains carried blaCTX-M genes. The CTX-M-9 group (56.0%, 70/125) was predominant, followed by the CTX-M-1 group (38.4%). Four strains harbored both CTX-M-1 group genes and CTX-M-9 group genes. The predominant genotypes of CTX-Menzyme were CTX-M-14 and CTX-M-15. Conclusion -lactam resistant strains harboring CTX-M type ESBLs were commonly found from healthy people engaged in food processing. Considering their close contact with food,more attention should be paid to the high risk of spreading these ESBLs resistant bacteria during their working process.

     

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