袁梦, 袁月明, 罗锦雁, 陈宏彬, 胡鹏威, 段永翔. 金黄色葡萄球菌毒力基因检测及Spa分型研究[J]. 疾病监测, 2016, 31(7): 603-608. DOI: 10.3784/j.issn.1003-9961.2016.07.015
引用本文: 袁梦, 袁月明, 罗锦雁, 陈宏彬, 胡鹏威, 段永翔. 金黄色葡萄球菌毒力基因检测及Spa分型研究[J]. 疾病监测, 2016, 31(7): 603-608. DOI: 10.3784/j.issn.1003-9961.2016.07.015
YUAN Meng, YUAN Yue-ming, LUO Jin-yan, CHEN Hong-bin, HU Peng-wei, DUAN Yong-xiang. Virulence gene detection and Spa typing of Staphylococcus aureus[J]. Disease Surveillance, 2016, 31(7): 603-608. DOI: 10.3784/j.issn.1003-9961.2016.07.015
Citation: YUAN Meng, YUAN Yue-ming, LUO Jin-yan, CHEN Hong-bin, HU Peng-wei, DUAN Yong-xiang. Virulence gene detection and Spa typing of Staphylococcus aureus[J]. Disease Surveillance, 2016, 31(7): 603-608. DOI: 10.3784/j.issn.1003-9961.2016.07.015

金黄色葡萄球菌毒力基因检测及Spa分型研究

Virulence gene detection and Spa typing of Staphylococcus aureus

  • 摘要: 目的 研究深圳市南山区2009-2015年医院内患者临床检测标本、医院台面与医护人员双手涂抹样以及食物中毒样本中金黄色葡萄球菌(Staphylococcus aureus,SA)蛋白A基因多态性分型(single locus DNA-sequencing of the repeat region of the Staphylococcus protein A gene,Spa)的特征及毒力基因分布情况。方法 采用多重PCR方法检测SA的mecA与femA基因,PCR方法检测24个毒力基因以及SA蛋白A基因,PCR产物测序后序列上传数据库进行比对。结果 87株SA共检出48株耐甲氧西林金黄色葡萄球菌(methicillin-resistant S. aureus,MRSA),检出率为55.2%。共检出21种毒力基因,检出率60%的毒力基因有LUKDE(65.5%)、SHE(67.8%)、mpHLG 2(85.1%)、HLD(93.1%)和 HLA(100.0%)。94.3%菌株携带5个以上毒力基因。三类样本中有11种毒力基因检出率差异有统计学意义,分别是SEB、mpSEC、mpSEJ、mpSEO、TST、HLB、HLD、mpHLG 1、mpHLG 2,mpETB和PVL。其中mpETB与PVL仅在患者中检出,肠毒素基因在食物中毒株中携带率较高。MRSA与甲氧西林敏感金黄色葡萄球菌(methicillin-sensitive S.aureus,MSSA)的3种毒力基因检出率差异有统计学意义,分别是mpETA、PVL及mpHLG 2;MRSA的毒力基因mpETA与PVL检出率明显高于MSSA(P=0.033, P=0.030),而mpHLG 2检出率明显低于MSSA(P=0.021)。87株SA分为24种Spa型,患者优势型别为t030(30.0%)与t437(36.7%),医院其他样本优势型别为t091(50.0%),食物中毒优势型别为t127(36.8%)与t091(21.1%)。结论 三类样本部分毒力基因检出差异有统计学意义。MRSA与MSSA携带毒力基因差异无统计学意义。三类样本中均存在优势的Spa型别。

     

    Abstract: Objective To study the distribution of virulence genes and the Spa types of Staphylococcus aureus isolated from patients, smears of material surface and medical workers' hands and samples from food poisoning cases in Nanshan district in Shenzhen from 2009 to 2015. Methods The mecA and femA genes of S.aureus were detected by multiplex polymerase chain reaction (PCR). Twenty four kinds of virulence genes and protein A gene of S.aureus were detected by PCR, the products of PCR were sequenced and the results were aligned with those in Spa database. Results Among 87 S.aureus strains,48(55.2%) were methicillin-resistant S. aureus (MRSA). Twenty one virulence genes were detected, the virulence genes with detection rate 60% included LUKDE(65.5%), SHE(67.8%), mpHL G2(85.1%),HLD(93.1%), HLA(100.0%). Up to 94.3% of the strains carried more than 5 virulence genes. The positive rates of 11 of kinds virulence genes(SEB,mpSEC, mpSEJ, mpSEO,TST, HLB, HLD, mpHL G1, mpHL G2, mpETB, PVL) were different. MpETB and PVL genes were detected only in patient samples.The positive rate of enterotoxins in food poisoning samples was higher.The positive rates of 3 kinds of virulence genes(mpETA,PVL,mpHL G2) were significantly different between MRSA and methicillin-sensitive S.aureus (MSSA). The positive rates of mpETA and PVL were higher (P=0.033, P=0.030), but the positive rate of mpHL G2 was lower in MRSA strains (P=0.021). Among the 87 strains,24 Spa types were identified. t437 and t030 were the top two Spa types of S.aureus strains from patients, accounting for 36.7% and 30.0% respectively, t091 was the predominant Spa type of S.aureus from smears of material surfaces and medical workers' hands, accounting for 50.0% and t127 and t091 were the top two Spa types of S.aureus from food poisoning samples, accounting for 36.8% and 21.1% respectively. Conclusion The positive rates of virulence genes in the S.aureus strains from 3 kinds of samples were significantly different, but the positive rates of virulence genes in MRSA and MSSA had no significant difference. There were predominant Spa types in the S.aureus strains from 3 kinds of samples.

     

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