吕冰, 张新, 黄瑛, 霍达, 严寒秋, 王全意, 曲梅. 北京地区2010-2015年宋内志贺菌脉冲场凝胶电泳分型及耐药特征分析[J]. 疾病监测, 2016, 31(10): 870-874. DOI: 10.3784/j.issn.1003-9961.2016.10.016
引用本文: 吕冰, 张新, 黄瑛, 霍达, 严寒秋, 王全意, 曲梅. 北京地区2010-2015年宋内志贺菌脉冲场凝胶电泳分型及耐药特征分析[J]. 疾病监测, 2016, 31(10): 870-874. DOI: 10.3784/j.issn.1003-9961.2016.10.016
LYU Bing, ZHANG Xin, HUANG Ying, HUO Da, YAN Han-qiu, WANG Quan-yi, QU Mei. Pulsed field gel electrophoresis typing and drug resistance detection of Shigella sonnei isolated in Beijing, 2010-2015[J]. Disease Surveillance, 2016, 31(10): 870-874. DOI: 10.3784/j.issn.1003-9961.2016.10.016
Citation: LYU Bing, ZHANG Xin, HUANG Ying, HUO Da, YAN Han-qiu, WANG Quan-yi, QU Mei. Pulsed field gel electrophoresis typing and drug resistance detection of Shigella sonnei isolated in Beijing, 2010-2015[J]. Disease Surveillance, 2016, 31(10): 870-874. DOI: 10.3784/j.issn.1003-9961.2016.10.016

北京地区2010-2015年宋内志贺菌脉冲场凝胶电泳分型及耐药特征分析

Pulsed field gel electrophoresis typing and drug resistance detection of Shigella sonnei isolated in Beijing, 2010-2015

  • 摘要: 目的 分析北京市2010-2015年分离的259株宋内志贺菌脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)分型及耐药特征。方法 收集2010-2015年分离到的宋内志贺菌259株,经生化及血清型鉴定后,应用PFGE分型并用最小抑菌浓度法(minimal inhibition concentration,MIC)进行12种药物的耐药检测。结果 259株宋内志贺菌经PFGE图谱聚类分析后,发现宋内志贺菌有A、B两个优势簇,2010-2015年A簇菌株所占构成比逐年增高;耐药结果显示257株为耐药菌株,其中245株为多耐药菌株,占所有耐药菌株的95.33%。对头孢曲松(CRO)的耐药性有明显升高。结论 北京地区近年宋内志贺菌有较高的同源性,A簇菌株已经成为目前北京地区宋内志贺菌的优势簇。对三代头孢类药物CRO等耐药性上升可能与临床上大量应用三代头孢药物以及A簇菌株增多有关,多耐药谱的情况也更加复杂,提示应合理使用抗生素。

     

    Abstract: Objective To understand the molecular and drug resistant characteristics of Shigella sonnei isolated in Beijing from 2010 to 2015. Methods A total of 259 Shigella sonnei strains isolated in Beijing from 2010 to 2015 were tested by pulsed field gel electrophoresis (PFGE) and the minimum inhibitory concentration of 12 antibiotics to the strains were detected after the serological and biochemical identification. Results PFGE results showed that the strains shared high homology (90%) and cluster A and B were the two major subtypes. The proportion of the strains in cluster A increased from 2010 to 2015. There were 257 drug resistant strains and 245 multidrug resistant strains (95.33%). It was noticeable that the drug resistant rate to ceftriaxone was in increase. Conclusion Shigella sonnei isolated in Beijing from 2010 to 2015 shared high homology, and cluster A was the predominant subtype. The increased rate of drug resistance to ceftriaxone might be related with the common clinical use of three-generation cephalosporin and increase of the strains in cluster A. Multidrug resistance was more complex, suggesting the importance of rational use of antibiotics.

     

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