戴映雪, 杜训波, 杨磊, 岳勇, 张晓春, 韩德琳. 冬春季节成都市活禽市场禽流感病毒污染情况及其影响因素分析[J]. 疾病监测, 2017, 32(2): 135-140. DOI: 10.3784/j.issn.1003-9961.2017.02.013
引用本文: 戴映雪, 杜训波, 杨磊, 岳勇, 张晓春, 韩德琳. 冬春季节成都市活禽市场禽流感病毒污染情况及其影响因素分析[J]. 疾病监测, 2017, 32(2): 135-140. DOI: 10.3784/j.issn.1003-9961.2017.02.013
DAI Ying-xue, DU Xun-bo, YANG Lei, YUE Yong, ZHANG Xiao-chun, HAN De-lin. Contamination of avian influenza virus in alive poultry markets and related factors in winter and spring in Chengdu[J]. Disease Surveillance, 2017, 32(2): 135-140. DOI: 10.3784/j.issn.1003-9961.2017.02.013
Citation: DAI Ying-xue, DU Xun-bo, YANG Lei, YUE Yong, ZHANG Xiao-chun, HAN De-lin. Contamination of avian influenza virus in alive poultry markets and related factors in winter and spring in Chengdu[J]. Disease Surveillance, 2017, 32(2): 135-140. DOI: 10.3784/j.issn.1003-9961.2017.02.013

冬春季节成都市活禽市场禽流感病毒污染情况及其影响因素分析

Contamination of avian influenza virus in alive poultry markets and related factors in winter and spring in Chengdu

  • 摘要: 目的 了解冬春季节成都市活禽市场中禽流感病毒污染情况,探索其影响因素,为禽流感防控提供科学依据。方法 在成都市5个区(县)的活禽市场开展采样监测,对宰杀点经营者进行面对面问卷调查,采用实时荧光定量聚合酶链反应方法对标本进行A型、H5亚型、H7亚型和H9亚型禽流感病毒核酸检测,采用2检验比较定性资料,采用二元logistic回归分析影响因素。结果 收集并检测环境标本255份,禽流感病毒A型阳性率51.0%(130/255),H5亚型阳性率11.0%(28/255),H9亚型阳性率22.4%(57/255),H5+H9亚型交叉阳性率9.8%(25/255),未分型阳性率7.8%(20/255),未检出H7亚型。2月A型、H9亚型、H5+H9亚型和未分型阳性率较3月更高(P0.05),使用脱毛机的A型、H5亚型、H9亚型阳性率较未使用的高(P0.05),批发市场的未分型阳性率较零售市场高(P0.05),专门收集禽血的宰杀点较将禽血直接排入下水道的未分型阳性率高(P0.05)。分别有6.4%(5/78)和11.5%(9/78)的经营者在操作时佩戴口罩和手套。结论 采取改良宰杀作业方式,严格落实清洁消毒、定期休市和健康教育等有关措施,从市场监管部门、市场管理方和宰杀点经营者三方面着手预防人感染禽流感病毒疫情发生。

     

    Abstract: Objective To understand the contamination of avian influenza virus in alive poultry markets in winter and spring in Chengdu and identify the related factors, so as to provide scientific evidence for the prevention and control of human infection with avian influenza virus. Methods Sampling was conducted in alive poultry markets of 5 districts and counties, and face to face questionnaire survey was conducted among the stalls owners. RT-PCR was used to detect A, subtype H5, H7 and H9 avian influenza virus nucleic acid by using the samples collected and 2 test and Binary logistic regression analysis were performed. Results A total of 255 samples were collected and detected. The positive rate of avian influenza A virus was 51.0%(130/255), while the positive rates of H5, H9, cross contamination of H5 and H9 and other subtype viruses were 11.0%(28/255), 22.4%(57/255), 9.8%(25/255), and 7.8%(20/255) respectively. No subtype H7 virus positive samples were found. Influential factors analysis showed that the positive rate of A,subtype H9, cross contamination of H5 and H9, and other subtype viruses were higher in February than in March(P0.05). Stalls with depilating machines had a higher positive rate of A, subtype H5 and H9 viruses than those without such machines(P0.05). The positive rates of other subtype viruses were higher in stalls collecting poultry blood for sale than those discharging poultry blood into sewage and in wholesale markets than in retail markets, respectively (P0.05). 6.4%(5/78) of the owners wore masks during the operation, and 11.5%(9/78) of them wore gloves. Conclusion To prevent human infection with avian influenza virus, it is necessary to improve the operation procedure of slaughtering, conduct regular disinfection and market suspension as well as health education.

     

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