Abstract: Objective To understand the infection status of HBV, HCV, Treponema pallidum and HIV in nameless patients in Hangzhou. Methods The serum HBsAg, anti-HCV, anti-TP and anti-HIV of 595 nameless patients treated in our hospital during January-December 2015 were tested and analyzed. Results Among the 595 patients, there were 63 were HBsAg positive (10.59%), 19 were anti-HCV positive (3.19%), 29 were anti-TP positive (4.87%) and 4 were anti-HIV positive (0.67%). Compared with the control group (normal in-patients), the differences in anti-HCV positive rate and anti-TP positive rate were significant (2=5.765, P=0.016; 2=14.053, P=0.001), but there were no gender and age specific significant differences in the positive rates of four serologic markers. Conclusion Detections of serum HBsAg, anti-HCV, anti-TP and anti-HIV in nameless patients is important to reduce the medical disputes and reduce the risk of occupational exposure in physicians.