Abstract: Objective To analyze the loss coefficient of bivalent live attenuated oral poliomyelitis vaccine (bOPV) and understand the loss extent of bOPV in Fengtai district of Beijing for the development of evidence based vaccine use plan and standardization of vaccine management. Methods A standardized daily reporting sheet of bOPV vaccination was used to collect the information about bOPV use in all vaccination clinics in Fengtai in May 2016. Results There were 67 vaccination clinics in Fengtai. From 9th to 27th in May 2016, the overall loss coefficient of bOPV was 2.10, the loss coefficient was 2.27 in village/military vaccination clinics, 2.22 in enterprise vaccination clinics and 1.98 in clinics of health department, the differences were significant. The difference in loss coefficient between vaccination clinics at lower level (2.24) and vaccination clinics at higher level (2.04) had significant difference. The loss coefficient was higher in clinics with 10 vaccination services daily (2.39) than in clinics with 10-20 vaccination services daily (2.12) and with 20 vaccination services (1.75), the difference was significant. Sharing dropper or not resulted in different loss coefficient, the difference was significant. Disusing vaccine after 30 minute cold storage and continuing to use vaccine also resulted in different loss coefficient, the difference was significant. Conclusion The loss coefficient of was high in Fengtai. In order to reduce the waste of vaccine, it is necessary to use dropper and remaining vaccine according to related regulations, conduct mass vaccination and reduce the unnecessary opening the package of bOPV.