Abstract: Objective To investigate the serotype, antibiotic resistance and molecular characteristics of Vibrio parahaemolyticus strains isolated from a food poisoning event in a company in Jiangmen of Guangdong province in 2017. Methods A total of 15 V. parahaemolyticus strains isolated from this food poisoning event were serotyped,tested for antibiotic resistance and detected for the tdh(thermostable direct hemolysin gene),trh(tdh-related hemolysin gene),GS-PCR and orf8 genes by PCR. Then they were all analyzed by using pulsed field gel electrophoresis(PFGE). Results The biochemical identification and serotyping results indicated all the 15 isolates were V. parahaemolyticus O3:K6 strains. The antibiotic susceptibility analysis showed that all the 15 strains were resistant to ampicillin and cefazolin,but sensitive to other antibiotics. Virulence gene detection suggested that all the 15 isolates were tdh positive,but trh negative,and carried GS-PCR and orf8 genes. The PFGE molecular typing of the strains digested by NotⅠand SfiⅠ showed that the finger-pattern of 14 strains from the cases and 1 strain from the food sample shared high similarity. Conclusion The pathogen of this food poisoning was V. parahaemolyticus O3:K6,which carried tdh,GS-PCR and orf8 genes and shared the common antibiotic resistance phenotype and genetic characteristics. The food poisoning was caused by two clones of V. parahaemolyticus.