梁胜楠, 姜祥坤, 杜银菊, 周璞, 刘莉, 程利红, 王多春. 2016年山东省聊城市临床病例中2种罕见施万菌的鉴定[J]. 疾病监测, 2019, 34(9): 817-821. DOI: 10.3784/j.issn.1003-9961.2019.09.010
引用本文: 梁胜楠, 姜祥坤, 杜银菊, 周璞, 刘莉, 程利红, 王多春. 2016年山东省聊城市临床病例中2种罕见施万菌的鉴定[J]. 疾病监测, 2019, 34(9): 817-821. DOI: 10.3784/j.issn.1003-9961.2019.09.010
Shengnan Liang, Xiangkun Jiang, Yinju Du, Pu Zhou, Li Liu, Lihong Cheng, Duochun Wang. Isolation of two rare Shewanella spp. from specimens of patients in Liaocheng in 2016[J]. Disease Surveillance, 2019, 34(9): 817-821. DOI: 10.3784/j.issn.1003-9961.2019.09.010
Citation: Shengnan Liang, Xiangkun Jiang, Yinju Du, Pu Zhou, Li Liu, Lihong Cheng, Duochun Wang. Isolation of two rare Shewanella spp. from specimens of patients in Liaocheng in 2016[J]. Disease Surveillance, 2019, 34(9): 817-821. DOI: 10.3784/j.issn.1003-9961.2019.09.010

2016年山东省聊城市临床病例中2种罕见施万菌的鉴定

Isolation of two rare Shewanella spp. from specimens of patients in Liaocheng in 2016

  • 摘要:
    目的对2016年山东省聊城市临床患者分离株进行鉴定以及分析其生化特性和药物敏感性。
    方法对患者来源标本及6株菌株进行选择性培养基分离培养,并进行生化试验、溶血试验、耐盐耐热实验和药敏试验,使用API生化鉴定系统、VITEK 2 Compact、全自动快速微生物质谱检测系统进行鉴定,并进行16S rRNA和gyrB序列分析。
    结果8株菌在菌落形态和生化特性上差异无统计学意义,只有溶血状态有所不同,临床鉴定系统有局限性,只能分辨到海藻施万菌和腐败施万菌,进一步使用16S rRNA和gyrB序列检测方法,确认患者分离株属于2种罕见的施万菌,即鲍施万菌和优佩内施万菌。
    结论首次在中国大陆的临床病例中,鉴定了2株由罕见施万菌导致的感染病例。 由施万菌属引起的临床感染还需要进一步重视,并加强监测。

     

    Abstract:
    ObjectiveTo identify the isolates of Shewanella spp. from two patient specimens in Liaocheng and explore the biochemical characteristics and drug susceptibility of the Shewanella spp.
    MethodsTwo specimens from the two patients and other 6 Shewanella spp. strains revived were cultured by using selective medium, and biochemical test, hemolytic test, salt-resistant and heat-resistant tests, drug-resistance test were conducted for the strains. API and VITEK 2 Compact, VITEK MS systems were used for the identification of the strains, 16S rRNA and gyrB sequence analyses were conducted.
    ResultsThe morphology and biochemical characteristics of 8 strains were consistent, the hemolytic state was different. The clinical identification system could only identified Shewanella algae and Shewanella putrefaciens. Two strains isolated from the patients were further identified as rare Shewanella haliotis and Shewanella upenei through 16S rRNA and gyrB gene sequence analyses.
    ConclusionIt was the first time to isolate rare Shewanella spp. from clinical cases in China. Further attention needs to be paid to clinical infection caused by Shewanella and the surveillance should be strengthened.

     

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