曹蓝, 李魁彪, 夏丹, 刘艳慧, 陈艺韵, 陆剑云, 张周斌, 狄飚. 2017-2019年广州市禽类市场H9N2禽流感病毒全基因组遗传进化特征分析[J]. 疾病监测, 2020, 35(3): 206-211. DOI: 10.3784/j.issn.1003-9961.2020.03.007
引用本文: 曹蓝, 李魁彪, 夏丹, 刘艳慧, 陈艺韵, 陆剑云, 张周斌, 狄飚. 2017-2019年广州市禽类市场H9N2禽流感病毒全基因组遗传进化特征分析[J]. 疾病监测, 2020, 35(3): 206-211. DOI: 10.3784/j.issn.1003-9961.2020.03.007
Lan Cao, Kuibiao Li, Dan Xia, Yanhui Liu, Yiyun Chen, Jianyun Lu, Zhoubin Zhang, Biao Di. Genetic evolution of avian influenza A(H9N2) viruses from poultry markets in Guangzhou, 2017–2019[J]. Disease Surveillance, 2020, 35(3): 206-211. DOI: 10.3784/j.issn.1003-9961.2020.03.007
Citation: Lan Cao, Kuibiao Li, Dan Xia, Yanhui Liu, Yiyun Chen, Jianyun Lu, Zhoubin Zhang, Biao Di. Genetic evolution of avian influenza A(H9N2) viruses from poultry markets in Guangzhou, 2017–2019[J]. Disease Surveillance, 2020, 35(3): 206-211. DOI: 10.3784/j.issn.1003-9961.2020.03.007

2017-2019年广州市禽类市场H9N2禽流感病毒全基因组遗传进化特征分析

Genetic evolution of avian influenza A(H9N2) viruses from poultry markets in Guangzhou, 2017–2019

  • 摘要:
    目的对H9N2禽流感病毒分离株进行全基因进化特征分析,为禽流感的防控提供研究数据。
    方法对24株2017—2019年广州市禽类市场H9N2禽流感毒株进行测序,通过生物信息学软件,分析全基因组遗传进化特点。
    结果24株H9N2病毒核苷酸同源性为86.20%~100.00%,核苷酸进化距离为0.00~1.54,其中HA基因的核苷酸和氨基酸进化距离变化最大,其次为PB2基因。 遗传进化分析显示,所有毒株各基因在系统发生树上聚为一簇,属于G57基因型。 分子特征分析显示,所有毒株属于低致病性禽流感病毒,在HA蛋白受体结合位点均发生Q226L突变,提示H9N2更容易识别人源受体。 在HA-N166D、PB2-L89V/292V、PB1-I368V、L473V、PA-K356R、S409N、M1-N30D、T215A、NS1-P42S等致病性相关位点上,大部分毒株发生致病性增强突变。 耐药性上,所有毒株对神经氨酸酶抑制剂敏感,对烷胺类药物耐药。
    结论2017—2019年期间广州市禽类市场H9N2禽流感病毒为G57基因型,该基因型病毒普遍发生与人受体结合能力增强的稳定变异,且大部分毒株发生致病性增强的突变,需加强监测。

     

    Abstract:
    ObjectiveTo analyze the characteristics of genetic evolution of avian influenza A (H9N2) virus strains isolated in Guangzhou and provide research data for the prevention and control of human infection with avian influenza virus.
    MethodsA total of 24 strains of H9N2 virus isolated from the samples collected in poultry markets in Guangzhou were randomly selected for sequencing. The genetic evolution characteristics of the strains were analyzed with bioinformatics software.
    ResultsThe nucleotide homology of all the strains ranged from 86.20% to 100.00%, and the evolutionary distance of nucleotide was 0.00–1.54. Genetic evolution analysis showed that all the strains clustered into same phylogenetic clades, and belonged to genotype G57. Molecular analysis showed that all the strains belonged to low pathogenic avian influenza viruses, and all strains had Q226L mutation in HA receptor binding sites, which suggested that these viruses can easily recognize the receptor of human. Most of the strains had mutations related to enhanced pathogenicity at the sites of HA-N166D, PB2-L89V/292V, PB1-I368V, L473V, PA-K356R, S409N, M1-N30D, T215A, NS1-P42S. All the strains were sensitive to neuraminidase inhibitors and resistant to alkylamines.
    ConclusionAvian influenza A (H9N2) viruses circulated in Guangzhou during 2017–2019 belonged to genotype G57, which had stable variation of enhanced binding ability to human receptor. Most of the strains had mutations of enhanced pathogenicity, which needed to be monitored continually.

     

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