舒高林, 李东迅, 彭华, 王维钧, 银安琪, 牛桓彩, 严寒秋. 2013-2019年北京市昌平区霍乱弧菌病原学和分子流行病学特征分析[J]. 疾病监测, 2020, 35(8): 735-741. DOI: 10.3784/j.issn.1003-9961.2020.08.013
引用本文: 舒高林, 李东迅, 彭华, 王维钧, 银安琪, 牛桓彩, 严寒秋. 2013-2019年北京市昌平区霍乱弧菌病原学和分子流行病学特征分析[J]. 疾病监测, 2020, 35(8): 735-741. DOI: 10.3784/j.issn.1003-9961.2020.08.013
Gaolin Shu, Dongxun Li, Hua Peng, Weijun Wang, Anqi Yin, Huancai Niu, Hanqiu Yan. Characteristics of phenotype and molecular type of Vibrio cholerae in Changping district of Beijing, 2013–2019[J]. Disease Surveillance, 2020, 35(8): 735-741. DOI: 10.3784/j.issn.1003-9961.2020.08.013
Citation: Gaolin Shu, Dongxun Li, Hua Peng, Weijun Wang, Anqi Yin, Huancai Niu, Hanqiu Yan. Characteristics of phenotype and molecular type of Vibrio cholerae in Changping district of Beijing, 2013–2019[J]. Disease Surveillance, 2020, 35(8): 735-741. DOI: 10.3784/j.issn.1003-9961.2020.08.013

2013-2019年北京市昌平区霍乱弧菌病原学和分子流行病学特征分析

Characteristics of phenotype and molecular type of Vibrio cholerae in Changping district of Beijing, 2013–2019

  • 摘要:
    目的了解2013 — 2019年北京市昌平区霍乱弧菌表型及其分子特征。
    方法采用常规培养方法对2013 — 2019年北京市昌平区疑似霍乱标本进行检测。 对分离鉴定的O1群小川型霍乱弧菌,用NotⅠ和SfiⅠ两种内切酶进行脉冲场凝胶电泳(PFGE)、用荧光PCR和普通PCR进行9种毒力基因检测,用微量肉汤稀释法进行21种药物敏感试验。
    结果2013 — 2019年北京市昌平区共分离到29株O1群小川型霍乱弧菌,其中散发病例中分离到10株,一起聚集性疫情中分离到19株。 29株菌经Not Ⅰ和Sfi Ⅰ双酶切后可分成3种PFGE谱型(CP-01~03),3种PFGE谱型间的相似系数为78.50%~100.00%,以CP-02为主(27/29,93.10%)。 所有菌株均不携带ctxAB基因,携带toxRhlyA基因,其他毒力基因ompUtcpAtcpIrtxCzotace的携带率分别为89.66%、82.76%、82.76%、44.83%、6.90%和6.90%。 29株菌药敏结果,除头孢唑啉(0.00%)和链霉素(3.45%)敏感率极低外,对其他19种抗菌素为敏感(82.76%~100.00%)。
    结论2013 — 2019年北京市昌平区的O1群小川型霍乱弧菌为非产毒株,以PFGE谱型CP-02为主,毒力基因toxRhlyA携带率高,对多数抗生素敏感,提示在防控工作中应关注此类菌株并制定相应的方针政策,减少疫情的发生。

     

    Abstract:
    ObjectivesTo understand the characteristics of the phenotype and molecular type of Vibrio cholerae strains in Changping district of Beijing from 2013 to 2019.
    MethodsThe conventional test was used for the detection of V. cholerae in suspected samples collected in Changping during this period. All V. cholerae O1 serotype Ogawa strains isolated were digested by two restrict enzyme (Not Ⅰ and SfiⅠ), and then analyzed with pulsed field gel electrophoresis. Nine virulence genes were detected by real-time PCR and PCR. The strains’ susceptibilities to 21 antibiotics were tested by minimal inhibitory concentration.
    ResultsA total of 29 V. cholerae O1 serotype Ogawa strains (10 strains isolated from sporadic cases, 19 strains isolated from an outbreak) were isolated in Changping from 2013 to 2019. Among the 29 V. cholerae strains, three PFGE patterns (CP-01–03) were found with the similarity coefficient from 78.5% to 100%, and the CP-02 (27/29, 93.10%) was the predominant PFGE pattern. The gene toxR and hlyA were found in all the strains, but no ctxAB was found. The carriage rates of gene ompU, tcpA, tcpI, rtxC, zot and ace were 89.66%, 82.76%, 82.76%, 44.83%, 6.90% and 6.90%, respectively. Except cefazolin (0.00%) and streptomycin (3.45%), most V. cholerae strains were sensitive to the other 19 antibiotics (82.76%–100.00%).
    ConclusionsAll the V. cholerae O1 serotype Ogawa strains isolated in Changping from 2013 to 2019 were non-toxigenic. Most V. cholerae O1 serotype Ogawa strains belonged to the same PFGE pattern. The carriage rates of gene toxR and hlyA were higher. The strains showed sensitivity to most antibiotics. Our data suggested that more attention should be paid to such strains and targeted measure are necessary in the future prevention and control to reduce cholera outbreak.

     

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