黄邵军, 袁珊珊, 诸佳辉. 浙江省余姚市猪肉和鸡肉中沙门菌的多样性及耐药性分析[J]. 疾病监测, 2022, 37(3): 396-400. DOI: 10.3784/jbjc.202105070243
引用本文: 黄邵军, 袁珊珊, 诸佳辉. 浙江省余姚市猪肉和鸡肉中沙门菌的多样性及耐药性分析[J]. 疾病监测, 2022, 37(3): 396-400. DOI: 10.3784/jbjc.202105070243
Huang Shaojun, Yuan Shanshan, Zhu Jiahui. Species diversity and antimicrobial resistance of Salmonella detected in pork and chicken in Yuyao, Zhejiang[J]. Disease Surveillance, 2022, 37(3): 396-400. DOI: 10.3784/jbjc.202105070243
Citation: Huang Shaojun, Yuan Shanshan, Zhu Jiahui. Species diversity and antimicrobial resistance of Salmonella detected in pork and chicken in Yuyao, Zhejiang[J]. Disease Surveillance, 2022, 37(3): 396-400. DOI: 10.3784/jbjc.202105070243

浙江省余姚市猪肉和鸡肉中沙门菌的多样性及耐药性分析

Species diversity and antimicrobial resistance of Salmonella detected in pork and chicken in Yuyao, Zhejiang

  • 摘要:
      目的   了解沙门菌在浙江省余姚市猪肉和鸡肉中的污染情况。
      方法   2019年6月至2020年10月采集余姚市城区主要商超和农贸市场生鲜猪肉和鸡肉共206份,对样本进行增菌,对分离的菌株进行鉴定、血清分型及药敏试验。
      结果   共采集61份鸡肉样本和145份猪肉样本,其中从25份鸡肉样本和68份猪肉样本分离到沙门菌,分离率分别为40.98%和46.90%。 93份肉类共分离到不同血清型沙门菌117株,其中来自猪肉样本的89株,来自鸡肉样本的28株。 这些菌株共分为17个血清型,在猪肉样品中,最常见的血清型是德尔卑沙门菌(30/89,33.71%)、伦敦沙门菌(15/89,16.85%)、里森沙门菌(14/89,15.73%)和鼠伤寒沙门菌(13/89,14.61%);而在鸡肉样品中,最常见的是肠炎沙门菌(9/28,32.14%)、肯塔基沙门菌(6/28,21.43%)和鼠伤寒沙门菌(4/28,14.29%)。 药敏试验结果显示,117株菌株对青霉素类、四环素类和叶酸途径阻断类抗生素表现出较高的耐药性,其中对氨苄西林、哌拉西林、四环素、复方新诺明的耐药率分别为79.49%、52.14%、74.36%、56.41%。 检出多重耐药(MDR)菌株71株,41株菌对氨苄西林–氯霉素–链霉素–复方新诺明–四环素耐药(ACSSuT),其中猪肉、鸡肉来源菌株分别为29、12株。 产超广谱β-内酰胺酶(ESBLs)菌株11株(9.40%),其中猪肉、鸡肉来源菌株分别为5(5.62%,5/89)、6株(21.43%,6/28)。
      结论   余姚市猪肉和鸡肉中沙门菌检出率高、菌型丰富、耐药严重、耐药机制复杂,应做好日常肉类的监测和分析工作,向有关部门反馈结果,及时进行干预,尽量减少沙门菌通过食物链传递给人类。

     

    Abstract:
      Objective  To understand the distribution and pollution of Salmonella in pork and chicken in Yuyao, Zhejiang.
      Methods  A total of 206 fresh pork and chicken samples were collected from major shopping malls and farm produce markets in Yuyao between June 2019 and October 2020, and then the enrichment, isolation, biochemical identification, serotyping and drug sensitivity test were conducted.
      Results  In 61 chicken samples and 145 pork samples, 25 and 68 were positive for Salmonella, respectively. The positive rates were 40.98% and 46.90% . A total of 117 strains of Salmonella belonging to 17 serotypes were isolated from 93 meat samples, including 89 strains from pork samples and 28 strains from chicken samples. S. derby (30/89, 33.71%), S. london (15/89, 16.85%), S. rissen (14/89, 15.73%) and S. typhimurium (13/89, 14.61%) were the predominant serotypes in pork samples. However, S. enteritidis (9/28, 32.14%), S. kentucky (6/28, 21.43%), and S. typhimurium (4/28, 14.29%) were most commonly detected in chicken samples. Severe antibiotic resistance to penicillins, tetracyclines and folate pathway antagonists were detected. The resistance rates to ampicillin, piperacillin and tetracycline and compound sulfamethoxazole were 79.49%, 52.14%, 74.36% and 56.41%, respectively. A total of 71 multidrug-resistance (MDR) strains were detected, and 41 strains, including 29 strains from pork samples and 12 strains from chicken samples, showed multi resistance to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole and tetracycline (ACSSuT). There were 11 extended-spectrum β-lactamases (ESBLs) producing strains, accounting for 9.40%, including 5 strains from pork samples (5.62%, 5/89) and 6 strains from chicken samples (21.43%, 6/28).
      Conclusion  This study indicated that the detection rate of Salmonella was high in chicken and pork samples in Yuyao. The isolates belonged to multi serotypes and showed high multidrug-resistance rate and complicated resistance mechanism. Therefore, it is necessary to strengthen the daily meat monitoring and analysis to provide evidence for the timely intervention to reduce transmission of Salmonella to humans through food chain.

     

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