杨晓晨, 陶晓燕, 何方玲, 王娟, 蔡亮, 戴纪强, 朱武洋. 湖南省狂犬病病毒种群划分情况及防控形势分析[J]. 疾病监测, 2022, 37(6): 764-768. DOI: 10.3784/jbjc.202111040574
引用本文: 杨晓晨, 陶晓燕, 何方玲, 王娟, 蔡亮, 戴纪强, 朱武洋. 湖南省狂犬病病毒种群划分情况及防控形势分析[J]. 疾病监测, 2022, 37(6): 764-768. DOI: 10.3784/jbjc.202111040574
Yang Xiaochen, Tao Xiaoyan, He Fangling, Wang Juan, Cai Liang, Dai Jiqiang, Zhu Wuyang. Population division and prevention and control situation of rabies virus in Hunan Province[J]. Disease Surveillance, 2022, 37(6): 764-768. DOI: 10.3784/jbjc.202111040574
Citation: Yang Xiaochen, Tao Xiaoyan, He Fangling, Wang Juan, Cai Liang, Dai Jiqiang, Zhu Wuyang. Population division and prevention and control situation of rabies virus in Hunan Province[J]. Disease Surveillance, 2022, 37(6): 764-768. DOI: 10.3784/jbjc.202111040574

湖南省狂犬病病毒种群划分情况及防控形势分析

Population division and prevention and control situation of rabies virus in Hunan Province

  • 摘要:
      目的  更新湖南省流行的狂犬病病毒(RABV)种群类型及进化特征,分析研判湖南省狂犬病疫情防控形势。
      方法  对湖南省2012—2017年收集的犬脑组织标本采用直接免疫荧光法或者反转录聚合酶链式反应法进行检测,对阳性标本进行N基因全序列(1 353 bp)测定及序列同源性分析,并与我国7个RABV种群的代表株一起进行系统发育分析。
      结果   新测的25株湖南省RABV毒株的N基因核苷酸同源性为91.9%~100%,氨基酸同源性为83.6%~100%;种群划分结果显示其中84%(21/25)的毒株为China Ⅰ群,4株为China Ⅱ群,未监测到2010年前在湖南流行的China Ⅲ群和China V群。
      结论  湖南省RABV流行种群类型未增加,且局限于我国两个主要的犬源种群。 切实加强犬的管理,尤其是农村犬的管理和免疫工作,才能进一步控制湖南省狂犬病疫情。

     

    Abstract:
      Objective  To update the population types and evolutionary characteristics of rabies virus (RABV) circulating in Hunan Province, and to analyze and judge the situation of rabies epidemic prevention and control in Hunan Province.
      Methods  The canine brain tissue specimens collected in Hunan Province from 2012 to 2017 were detected by direct immunofluorescence and reverse transcription polymerase chain reaction. The positive specimens were tested for the full sequence of N gene (1 353 bp) and sequence homology. Analyze and conduct phylogenetic analysis together with the representative strains of 7 RABV populations in China.
      Results  The N gene nucleotide homology of the 25 newly tested RABV strains in Hunan Province was 91.9%–100%, and the amino acid homology was 83.6%–100%; the results of population division showed that 84% (21/25) of the virus strains are ChinaⅠgroup, 4 strains are China Ⅱgroup, China Ⅲ group and China V group prevalent in Hunan before 2010 have not been detected.
      Conclusion  The type of rabies virus epidemic population in Hunan Province has not increased, and it is limited to the two main canine populations in China. Only by effectively strengthening dog management, especially the management and immunization of rural dogs, can the rabies epidemic in Hunan Province be further controlled.

     

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