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高祯, 龚利强, 李娜, 杜海军, 梅国勇, 杨燕清, 董鸿铭, 魏泽, 周正元, 韩俊. 江苏省常熟市一起聚集性儿童上呼吸道感染疫情的病原学调查[J]. 疾病监测, 2023, 38(3): 282-286. DOI: 10.3784/jbjc.202201050563
引用本文: 高祯, 龚利强, 李娜, 杜海军, 梅国勇, 杨燕清, 董鸿铭, 魏泽, 周正元, 韩俊. 江苏省常熟市一起聚集性儿童上呼吸道感染疫情的病原学调查[J]. 疾病监测, 2023, 38(3): 282-286. DOI: 10.3784/jbjc.202201050563
shu
Gao Zhen, Gong Liqiang, Li Na, Du Haijun, Mei Guoyong, Yang Yanqing, Dong Hongming, Wei Ze, Zhou Zhengyuan, Han Jun. Etiological investigation on the epidemic of upper respiratory tract infection among children in Changshu, Jiangsu[J]. Disease Surveillance, 2023, 38(3): 282-286. DOI: 10.3784/jbjc.202201050563
Citation: Gao Zhen, Gong Liqiang, Li Na, Du Haijun, Mei Guoyong, Yang Yanqing, Dong Hongming, Wei Ze, Zhou Zhengyuan, Han Jun. Etiological investigation on the epidemic of upper respiratory tract infection among children in Changshu, Jiangsu[J]. Disease Surveillance, 2023, 38(3): 282-286. DOI: 10.3784/jbjc.202201050563
shu

江苏省常熟市一起聚集性儿童上呼吸道感染疫情的病原学调查

Etiological investigation on the epidemic of upper respiratory tract infection among children in Changshu, Jiangsu

    摘要
  • 摘要:
      目的   分析2021年9月江苏省常熟市某幼儿园一起急性上呼吸道感染聚集性疫情发生的原因。
      方法   收集2021年9月江苏省常熟市某幼儿园急性上呼吸道感染患儿的临床信息，采集患儿咽拭子样本。采用实时荧光定量PCR对样本进行18种呼吸道病原体筛查。利用巢式PCR法对高检出率的鼻病毒进行分型片段扩增及测序。 使用MEGA 7.0软件对测序结果进行序列比对，并构建系统进化树。
      结果   此次聚集性急性上呼吸道感染疫情主要临床症状为流涕、咽痛。 共采集患儿咽拭子样本51份，其中男童24份、女童27份。18种呼吸道病原体筛查结果显示，检出率为80.39%（41/51），其中单一病毒检出率为64.71%（33/51），混合病毒检出率为15.69%（8/51）。 检出的单一病毒中鼻病毒占比最高，为42.43%（14/33）。 其他病毒占比依次为细小病毒B19占18.18%（6/33），博卡病毒为15.15%（5/33），冠状病毒229E为6.06%（2/33），人偏肺病毒、乙型流感病毒、腺病毒、呼吸道合胞病毒、冠状病毒OC43和NL63均为3.03%（1/33）。 7例鼻病毒与其他病毒混合感染。对21份鼻病毒阳性样本进行VP4-VP2基因片段扩增和测序，12株成功分型，均为A组，其中血清型A19、A22、A41各1株，血清型A49共9株。
      结论   本次急性呼吸道感染病例的咽拭子中检出多种呼吸道病毒，以鼻病毒居多，血清型A49与此次聚集性病例的发生密切相关。

     

    Abstract:
      Objective  To analyze the pathogen of an outbreak of acute respiratory tract infection(ARTI) in a kindergarten in Changshu, Jiangsu.
      Methods  In September 2021, throat swabs were collected from children with ARTI at a kindergarten in Changshu, Jiangsu, then 18 respiratory pathogens were screened on these samples. The VP4-VP2 gene of rhinovirus with the highest detection rate were amplified by nested PCR. After the genotype of rhinovirus was analyzed using software MEGA 7.0, the phylogenetic tree was constructed according to the sequence of VP4-VP2.
      Results  A total of 51 pharyngeal swabs were collected from pediatric patients, including 24 boys and 27 girls. The main clinical symptoms were runny nose and sore throat. The screening results of 18 respiratory pathogens showed that 41 samples were positive for virus, accounting for 80.39% (41/51). The detection rate of single virus was 64.71% (33/51), and the co-infection rate of viruses was 15.69% (8/51). The detection rate of rhinovirus was the highest in both single virus positive samples (42.43%,14/33) and co-infection samples(87.5%, 7/8). Other viruses, including parvovirus B19 (18.18%, 6/33), boca virus (15.15%, 5/33), coronavirus 229E (6.06%, 2/33), human metapneumovirus, influenza B virus, adenovirus, respiratory syncytial virus and coronavirus OC43 and NL63 (3.03%, 1/33), were also detected respectively. After 12 VP4-VP2 fragments from 21 rhinovirus positive samples were amplified, the sequence analysis showed that the 12 strains belonged to 4 serotypes of rhinovirus A including 9 strains of A49, 1 strains of A19, 1 strain of A22 and 1 strain of A41.
      Conclusion  Many viruses were detected in swabs samples from pediatric patients in an outbreak of ARTI in a kindergarten in Changshu. Rhinovirus A49 had the highest detection rate, which might be closely related to the outbreak of ARTI in a kindergarten in Changshu.

     

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