普通拟杆菌Bv46益生功能评估

乔蕾; 徐明超; 林晓颖; 张素平; 张桂; 杨晶; 孙晖; 刘丽云; 徐建国

doi:10.3784/jbjc.202202280075

普通拟杆菌Bv46益生功能评估

Evaluation of the probiotic properties of Bacteroides vulgatus Bv46

摘要

摘要:
目的评估普通拟杆菌（Bacteroides vulgatus）Bv46的生物特性和益生潜能。
方法模拟胃肠道的酸性和胆盐环境，评估普通拟杆菌Bv46耐逆性特点；通过溶血、药敏和明胶酶定性实验评估其安全性；基于其自聚集、疏水性和对HT-29细胞的黏附情况评估其黏附能力；利用琼脂扩散、色谱和吸光度测定，评估Bv46对致病菌的拮抗、产酸和抗氧化功能。
结果分离自健康人群的普通拟杆菌Bv46在模拟胃液和0.3%胆盐中的存活率分别为59.55%和63.76%；无溶血现象和明胶酶活性，除对氨苄西林和青霉素G耐药外，对其他所检测抗生素均敏感；Bv46具有中等自聚集能力、高疏水性和对HT-29细胞的中等黏附性；对致病性大肠埃希菌和鼠伤寒沙门菌的生长有抑制作用；此外，Bv46可以产生短链脂肪酸，并且具有抗氧化作用。
结论普通拟杆菌Bv46是潜在的益生菌。

Abstract:
Objective To evaluate probiotic potential of Bacteroides vulgatus Bv46.
Methods The Bv46 strain was tested on their tolerance to artificial gastric juices and bile salts. The Bv46 was assessed the hemolytic activity, antimicrobial susceptibility and gelatinase activity. Moreover, the probiotic properties of Bv46 were evaluated about auto-aggregation, hydrophobicity, adhesion to HT-29 cells, antimicrobial activity against pathogens, acid production and antioxidant properties.
Results It was 59.55% and 63.76% to the survival rates of Bv46 isolated from healthy people in simulated gastric juice and 0.3% bile salts respectively. Bv46 showed no haemolytic and gelatinase activities, and were only resistant to ampicillin and penicillin G. Bv46 showed medium auto-aggregation ability, but high hydrophobicity and medium adhesion to HT-29 cells. Moreover, Bv46 inhibited the growth of pathogenic Escherichia coli and Salmonella typhimurium. Furthermore, Bv46 produced short-chain fatty acids, and had antioxidant effects.
Conclusion The Bv46 is a potential probiotic.

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