郝彤宇, 马嘉嘉, 武雅蓉, 钱修伟, 张湘莉兰, 崔玉军. pre-CTXφ噬菌体在霍乱弧菌基因组中的遗传多样性研究[J]. 疾病监测, 2022, 37(11): 1403-1407. DOI: 10.3784/jbjc.202206120256
引用本文: 郝彤宇, 马嘉嘉, 武雅蓉, 钱修伟, 张湘莉兰, 崔玉军. pre-CTXφ噬菌体在霍乱弧菌基因组中的遗传多样性研究[J]. 疾病监测, 2022, 37(11): 1403-1407. DOI: 10.3784/jbjc.202206120256
Hao Tongyu, Ma Jiajia, Wu Yarong, Qian Xiuwei, Zhang Xianglilan, Cui Yujun. Genetic diversity of pre-CTXφ phages in Vibrio cholerae[J]. Disease Surveillance, 2022, 37(11): 1403-1407. DOI: 10.3784/jbjc.202206120256
Citation: Hao Tongyu, Ma Jiajia, Wu Yarong, Qian Xiuwei, Zhang Xianglilan, Cui Yujun. Genetic diversity of pre-CTXφ phages in Vibrio cholerae[J]. Disease Surveillance, 2022, 37(11): 1403-1407. DOI: 10.3784/jbjc.202206120256

pre-CTXφ噬菌体在霍乱弧菌基因组中的遗传多样性研究

Genetic diversity of pre-CTXφ phages in Vibrio cholerae

  • 摘要: 溶原性噬菌体CTXφ携带的ctxAB基因是霍乱弧菌产毒株最主要的毒力基因,而pre-CTXφ作为CTXφ的前体,缺失ctxAB基因编码序列。 基因组中整合了pre-CTXφ噬菌体的霍乱弧菌非产毒株,可通过其携带的zotace基因,引起患者轻度至中度的霍乱样腹泻症状,存在公共卫生风险。 本研究收集了公开发表的1649株霍乱弧菌草图序列和43株完成图序列,使用比较基因组学和群体遗传学方法,对上述霍乱弧菌数据集进行综合分析,结果表明:不同谱系霍乱弧菌基因组中pre-CTXφ的拷贝数存在差异;其中L3b.4菌株中pre-CTXφ拷贝数呈现随分离年份增加的趋势。 L2、L4、L7和L3b谱系存在pre-CTXφ与CTXφ共整合的现象,并发现pre-CTXφ位于pre-CTXφ/CTXφ噬菌体家族系统发育树的祖先位置。 这些发现进一步丰富了对霍乱弧菌基因组中pre-CTXφ/CTXφ噬菌体家族遗传多样性的认识,为阐明其整合机制和进化规律提供了必要基础,并为携带此类噬菌体霍乱弧菌的检测及防控提供借鉴。

     

    Abstract: pre-CTXφ phage is the precursor of phage CTXφ. Unlike CTXφ, pre-CTXφ does not include the cholera toxin coding genes, ctxAB, which are the most important virulence genes in Vibrio cholerae toxigenic strains. However, pre-CTXφ carries zot and ace genes. After pre-CTXφ integrates into non-toxigenic strains, the zot and ace genes can cause cholera-like diarrhea in humans and lead to potential public health risks. In this study, we collected 1649 publicly available draft genomes and 43 complete genomes of V. cholerae, and performed a comprehensive analysis of the genetic diversity of pre-CTXφ in V. cholerae genomes based on comparative genomics and population genetics. The results revealed that the copy numbers of pre-CTXφ vary among different phylogenetic lineages. Especially in L3b.4 lineage, the copy numbers of pre-CTXφ in strains showed an increasing trend over isolation years. We also found that co-integration of pre-CTXφ and CTXφ exists in strains from L2, L4, L7 and L3b lineages. Meanwhile, the phylogenetic tree of pre-CTXφ/CTXφ gene family showed that pre-CTXφ was located at the ancestral position. These findings enrich our knowledge about the integration and evolution of the pre-CTXφ phages in V. cholerae genomes, helping for the detection and infection control of Vibrio cholerae carrying pre-CTXφ.

     

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