李喆, 徐颖华, 辛晓芳. 我国钩端螺旋体分子分型研究进展[J]. 疾病监测, 2022, 37(4): 512-516. DOI: 10.3784/jbjc.202109130500
引用本文: 李喆, 徐颖华, 辛晓芳. 我国钩端螺旋体分子分型研究进展[J]. 疾病监测, 2022, 37(4): 512-516. DOI: 10.3784/jbjc.202109130500
Li Zhe, Xu Yinghua, Xin Xiaofang. Progress in research of molecular typing of Leptospira in China[J]. Disease Surveillance, 2022, 37(4): 512-516. DOI: 10.3784/jbjc.202109130500
Citation: Li Zhe, Xu Yinghua, Xin Xiaofang. Progress in research of molecular typing of Leptospira in China[J]. Disease Surveillance, 2022, 37(4): 512-516. DOI: 10.3784/jbjc.202109130500

我国钩端螺旋体分子分型研究进展

Progress in research of molecular typing of Leptospira in China

  • 摘要: 随着分子生物学、基因组学和生物信息学的发展,钩端螺旋体(钩体)分子流行病学的研究也愈发深入。 新的分子分型技术以及大量钩体基因组的测序,使得研究者们对钩体可以有一个全新的认识。 本研究以近年来国内外各种钩体分子分型方法为分类,总结并归纳了16S rRNA基因分型、脉冲场凝胶电泳分析、多位点序列分型、多位点可变数目串联重复序列分型和全基因组测序分析方法,有助于新的菌株型别的发现,揭示不同菌株间的宿主传播关系,地域传播关系,可为今后钩体分子分型方法的应用和钩体病的预防控制提供借鉴。

     

    Abstract: With the development of molecular biology, genomics and bioinformatics, the molecular epidemiology of Leptospira has been further studied. New molecular typing methods and the sequencing of a large number of Leptospira genomes have enabled researchers to gain a new understanding of Leptospira. Based on the molecular classification methods of Leptospira applied at home and abroad in recent years, this paper summarizes the 16S rRNA genotyping, pulsed field gel electrophoresis, site sequence classification, site variable number tandem repeat sequence classification and whole genome sequencing to help the finding of new type strains of Leptospira, and revealing the host and regional transmission relationship among different strains. This review can provide reference for the application of molecular typing methods and the prevention and control of leptospirosis in the future.

     

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