李崇山, 李智, 王静, 胡家瑜, 唐伟, 杨玉颖, 李云逸, 杨彦基, 崔晓娴, 叶玉龙, 张曦, 袁政安. 2018年上海市一起D8基因型麻疹病毒引起的输入性麻疹暴发报告[J]. 疾病监测, 2019, 34(7): 672-675. DOI: 10.3784/j.issn.1003-9961.2019.07.020
引用本文: 李崇山, 李智, 王静, 胡家瑜, 唐伟, 杨玉颖, 李云逸, 杨彦基, 崔晓娴, 叶玉龙, 张曦, 袁政安. 2018年上海市一起D8基因型麻疹病毒引起的输入性麻疹暴发报告[J]. 疾病监测, 2019, 34(7): 672-675. DOI: 10.3784/j.issn.1003-9961.2019.07.020
Chongshan Li, Zhi Li, Jing Wang, Jiayu Hu, Wei Tang, Yuying Yang, Yunyi Li, Yanji Yang, Xiaoxian Cui, Yulong Ye, Xi Zhang, Zhengan Yuan. Report of an imported measles outbreak caused by measles virus D8 in Shanghai, 2018[J]. Disease Surveillance, 2019, 34(7): 672-675. DOI: 10.3784/j.issn.1003-9961.2019.07.020
Citation: Chongshan Li, Zhi Li, Jing Wang, Jiayu Hu, Wei Tang, Yuying Yang, Yunyi Li, Yanji Yang, Xiaoxian Cui, Yulong Ye, Xi Zhang, Zhengan Yuan. Report of an imported measles outbreak caused by measles virus D8 in Shanghai, 2018[J]. Disease Surveillance, 2019, 34(7): 672-675. DOI: 10.3784/j.issn.1003-9961.2019.07.020

2018年上海市一起D8基因型麻疹病毒引起的输入性麻疹暴发报告

Report of an imported measles outbreak caused by measles virus D8 in Shanghai, 2018

  • 摘要:
    目的追踪2018年上海市一起输入性麻疹暴发的来源,并分析其病毒分子流行病学特征。
    方法直接从临床咽拭子标本中提取病毒核酸,采用一步法反转录–聚合酶链式反应,扩增麻疹病毒核蛋白基因COOH末端676个核苷酸,然后对扩增产物进行核苷酸序列测定,并分析其同源性,以确定病毒基因型及其可能的来源。
    结果本次暴发病例来自于同一家庭,且2例成年人病例在发病前21 d内有共同的出境旅游史;导致此次暴发的麻疹病毒为D8基因型。
    结论结合流行病学调查和实验室检测结果,推断此次疫情为一起D8基因型麻疹病毒引起的输入性麻疹暴发。

     

    Abstract:
    ObjectiveTo trace the source of an imported measles outbreak occurred in Shanghai in 2018, and analyze the molecular characteristics of the measles virus isolated.
    MethodsViral RNA was directly extracted from the throats swabs of the cases. The 676 nucleotides at COOH-terminal of nucleoprotein gene in measles virus were amplified by using one-step RT-PCR. The amplicons were sequenced by Sanger's to obtain the viral nucleotide sequences. Homology analysis was conducted to identify the viral genotype.
    ResultsThe cases in this outbreak came from a family, and 2 adult cases had a common outbound travel history within 21 d before the onset. The outbreak was caused by Measles virus D8.
    ConclusionBased on the results of epidemiologic investigation and laboratory tests, it was deduced that it was an imported measles outbreak caused by measles virus D8.

     

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