青藏高原岩羊朊蛋白基因及氨基酸序列分析

伍晶星 高利萍 陈冬冬 肖康 武月章 董小平 石琦

伍晶星, 高利萍, 陈冬冬, 肖康, 武月章, 董小平, 石琦. 青藏高原岩羊朊蛋白基因及氨基酸序列分析[J]. 疾病监测.
引用本文: 伍晶星, 高利萍, 陈冬冬, 肖康, 武月章, 董小平, 石琦. 青藏高原岩羊朊蛋白基因及氨基酸序列分析[J]. 疾病监测.
Wu Jingxing, Gao Liping, Chen Dongdong, Xiao Kang, Wu Yuezhang, Dong Xiaoping, Shi Qi. Analysis on sequences of PRNP and amino acid of blue sheep on Qinghai-Tibet Plateau[J]. Disease Surveillance.
Citation: Wu Jingxing, Gao Liping, Chen Dongdong, Xiao Kang, Wu Yuezhang, Dong Xiaoping, Shi Qi. Analysis on sequences of PRNP and amino acid of blue sheep on Qinghai-Tibet Plateau[J]. Disease Surveillance.

青藏高原岩羊朊蛋白基因及氨基酸序列分析

基金项目: 国家自然科学基金(No. 81630062);传染病预防控制国家重点实验室杰青申报资助(No. 2019SKLID501)
详细信息
    作者简介:

    伍晶星,男,浙江省台州市人,在读硕士,主要从事朊病毒研究工作,Email:441381438@qq.com

    通讯作者:

    石琦,Tel:010-58900818,E-mail:shiqi76@126.com

  • 中图分类号: R211; R373.9; R511

Analysis on sequences of PRNP and amino acid of blue sheep on Qinghai-Tibet Plateau

Funds: This study was supported by the Chinese National Natural Science Foundation (No. 81630062) and the State Key Laboratory for Infectious Disease Prevention and Control, China CDC (No. 2019SKLID501)
More Information
  • 摘要:   目的  分析岩羊的朊蛋白基因(prion protein gene,PRNP)序列,评估岩羊对羊瘙痒病的易感性。  方法  参照GenBank上已经发表的绵羊PRNP序列设计引物,然后对岩羊的PRNP序列进行扩增,基因测序后与已发表在GenBank上的11种哺乳动物的PRNP序列比对和分析。  结果  测序结果显示岩羊PRNP由771个核苷酸构成,编码256个氨基酸。序列分析结果显示4只岩羊PrP(prion protein,PrP)氨基酸序列完全同源,并且与野生型绵羊PrP氨基酸序列完全一致。此外,岩羊PrP氨基酸序列与山羊(99.2%)、驯鹿(98%)、牛(97.7%)也具有较高的同源性。  结论  本次测定的4只岩羊可能和野生型绵羊一样对羊瘙痒病中度易感,岩羊和绵羊种群之间存在相互传播羊瘙痒病的可能。
  • 图  1  PRNP序列PCR产物的凝胶电泳结果

    注:M:DL2000 DNA marker,-:阴性对照,,1-4:4只岩羊PRNP序列PCR扩增产物

    Figure  1.  Agarose gel electrophorsis result of PRNP PCR products

    图  2  12种哺乳动物PrP氨基酸序列比对结果

    注:岩羊(MW194319),绵羊(NM_001009481.1),山羊(NM_001314247.1),驯鹿(MN052820.1),牛(NM_181015.4),马(NM_001143798),犬(NM_001013423.1),兔子(NM_001082021.1),猫(XM_019826795.2),人(NM_000311.5),金黄地鼠(XM_005068660.3),小鼠(NM_011170.3)。相同的氨基酸位点用点表示,缺失的氨基酸位点用短线表示

    Figure  2.  Amino acid sequence alignment of prion proteins of 12 mammals

    图  3  12种哺乳动物PrP氨基酸序列系统发育树

    注:岩羊(MW194319),绵羊(NM_001009481.1),山羊(NM_001314247.1),牛(NM_181015.4),驯鹿(MN052820.1),犬(NM_001013423.1),猫(XM_019826795.2),马(NM_001143798),金黄地鼠(XM_005068660.3),小鼠(NM_011170.3),人(NM_000311.5),兔子(NM_001082021.1)。

    Figure  3.  Phylogenetic tree of prion proteins of 12 mammals

    表  1  12种哺乳动物PRNP序列同源性比较

    Table  1.   Nucleotide sequence homology of prion proteins genes of 12 mammals

    相似度(%)
    差异度(%)12345678910111213
    199.9100.099.797.897.389.688.488.485.985.284.183.11岩羊1
    20.199.999.697.797.189.788.588.586.085.484.383.22岩羊2
    30.00.199.797.897.389.688.488.485.985.284.183.13绵羊
    40.30.40.397.597.089.388.488.185.985.083.982.84山羊
    52.22.42.22.596.689.387.888.485.985.083.382.75
    62.82.92.83.13.589.187.688.085.784.884.383.56驯鹿
    711.411.211.411.711.712.089.290.089.285.886.885.17
    812.812.612.812.813.613.711.887.490.885.087.183.08
    912.712.612.713.012.713.210.813.986.687.787.186.39
    1016.015.916.016.016.016.211.89.914.985.488.484.510
    1116.716.516.717.017.017.115.916.813.616.485.890.311金黄地鼠
    1218.117.918.118.519.217.914.614.214.312.715.885.012兔子
    1319.619.419.620.020.219.016.919.615.317.710.517.013小鼠
    12345678910111213
      注:岩羊1(MW194319),岩羊2(MW194319的A567G替换),绵羊(NM_001009481.1),山羊(NM_001314247.1),驯鹿(MN052820.1),牛(NM_181015.4),马(NM_001143798),犬(NM_001013423.1),兔子(NM_001082021.1),猫(XM_019826795.2),人(NM_000311.5),金黄地鼠(XM_005068660.3),小鼠(NM_011170.3)
    下载: 导出CSV

    表  2  12种哺乳动物PrP氨基酸序列同源性比较

    Table  2.   Amino acid sequence homology of prion proteins of 12 mammals

    相似度(%)
    差异度(%)123456789101112
    1100.099.298.097.794.594.192.991.491.389.389.21岩羊
    20.099.298.097.794.594.192.991.491.389.389.22绵羊
    30.80.897.396.993.794.192.191.490.588.588.43山羊
    42.02.02.897.392.592.292.190.290.988.988.84驯鹿
    52.42.43.22.892.991.891.790.692.188.588.85
    65.75.76.67.97.492.290.590.289.786.587.36
    76.16.16.18.38.78.390.194.187.486.286.57
    87.57.58.48.48.810.210.688.289.788.988.98兔子
    99.19.19.110.510.010.56.112.986.685.484.99
    109.39.310.29.88.411.113.911.114.889.790.110
    1111.611.612.512.112.514.915.312.016.311.194.511金黄地鼠
    1211.611.612.612.112.114.014.912.116.910.75.812小鼠
    123456789101112
      注:岩羊(MW194319),绵羊(NM_001009481.1),山羊(NM_001314247.1),驯鹿(MN052820.1),牛(NM_181015.4),马(NM_001143798),犬(NM_001013423.1),兔子(NM_001082021.1),猫(XM_019826795.2),人(NM_000311.5),金黄地鼠(XM_005068660.3),小鼠(NM_011170.3)
    下载: 导出CSV
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  • 收稿日期:  2021-01-27
  • 网络出版日期:  2021-06-29

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