王佳奇, 顾一心, 周贵兰, 梁昊, 张建中, 张茂俊. 空肠弯曲菌脂寡糖核心区合成相关基因簇遗传多样性研究[J]. 疾病监测, 2020, 35(1): 11-15. DOI: 10.3784/j.issn.1003-9961.2020.01.005
引用本文: 王佳奇, 顾一心, 周贵兰, 梁昊, 张建中, 张茂俊. 空肠弯曲菌脂寡糖核心区合成相关基因簇遗传多样性研究[J]. 疾病监测, 2020, 35(1): 11-15. DOI: 10.3784/j.issn.1003-9961.2020.01.005
Jiaqi Wang, Yixin Gu, Guilan Zhou, Hao Liang, Jianzhong Zhang, Maojun Zhang. Genetic diversity of lipooligosaccharide core biosynthesis gene clusters in Campylobacter jejuni[J]. Disease Surveillance, 2020, 35(1): 11-15. DOI: 10.3784/j.issn.1003-9961.2020.01.005
Citation: Jiaqi Wang, Yixin Gu, Guilan Zhou, Hao Liang, Jianzhong Zhang, Maojun Zhang. Genetic diversity of lipooligosaccharide core biosynthesis gene clusters in Campylobacter jejuni[J]. Disease Surveillance, 2020, 35(1): 11-15. DOI: 10.3784/j.issn.1003-9961.2020.01.005

空肠弯曲菌脂寡糖核心区合成相关基因簇遗传多样性研究

Genetic diversity of lipooligosaccharide core biosynthesis gene clusters in Campylobacter jejuni

  • 摘要:
    目的分析我国空肠弯曲菌脂寡糖(LOS)核心区合成相关基因簇的序列特征,获得LOS核心区合成相关基因簇的特异DNA序列特征。
    方法选择132株空肠弯曲菌的全基因组序列,采用OrthoMCL软件,进行LOS核心区合成相关基因簇DNA的同源性分析并以特异型别基因簇的特异序列为靶位点,建立PCR方法,根据特异引物序列以及扩增产物大小,识别菌株LOS核心区合成相关基因簇的主要型别。
    结果在132株菌中,105株菌的LOS核心区合成相关基因簇的DNA序列属于14个已确定的LOS型别,27株菌为新型LOS,根据DNA的同源性可分为10个新型别,分别命名为CDC1~10。 LOS合成相关基因序列中存在大量的多聚A/T、多聚G/C结构以及碱基的插入、缺失等突变,部分突变有可能导致LOS相关合成酶及转移酶的氨基酸序列发生改变,影响其抗原性。 针对A、B、C、CDC8型LOS的特异DNA序列建立的PCR鉴定方法可在50株测序菌株的基因组中得到验证。
    结论10种LOS新型别的发现有利于更准确地鉴定空肠弯曲菌,解释LOS的抗原多样性特征。 空肠弯曲菌的LOS核心区合成相关基因簇序列多样丰富,存在大量多聚结构和DNA序列的突变,是潜在的导致LOS抗原多样性的原因。 主要LOS分型的PCR法可用于快速筛查5种特异LOS型,但仍需要更多菌株的验证。

     

    Abstract:
    ObjectiveTo analyze the DNA sequence characteristics of the biosynthesis gene clusters in the lipooligosaccharide (LOS) core region of Campylobacter jejuni, and obtain the specific sequence features of the LOS types.
    MethodsA total of 132 whole genome sequences of C. jejuni were selected for analyzing. OrthoMCL software was used to obtain the homology of LOS core region biosynthesis gene clusters and specific sequence of the different LOS type. PCR assay was established targeting the specific sequences of specific types of the gene clusters. According to the specific primers and the size of the amplified products, the main types of LOS were identified.
    ResultsAmong the 132 strains, the DNA sequences of biosynthesis gene clusters in LOS core regions of 105 strains belonged to 14 previously identified types, and those of 27 strains belonged to 10 novel types according to DNA homology, i.e. CDC1- 10. There were a large number of polyA/T, polyG/C, insertion and deletion mutations of nucleobase in the sequences of the LOS core region biosynthesis gene clusters. Some mutations could lead to the amino acid mutations of the synthetase or transferase for the LOSs, affecting the antigenicity. The type A, B, C, and CDC8 of LOS specific DNA sequences were confirmed in 50 sequenced C. jejuni strains using the PCR assays developed in this study.
    ConclusionThe 10 novel LOS types are helpful for the accurate identification of C. jejuni and explaining the antigenic diversity of LOS. The DNA sequence of LOS core biosynthesis gene clusters of C. jejuni is highly variable, and there are a large number of poly-structures and DNA sequence mutations, which is the potential cause of LOS antigenic diversity. The PCR assay for LOS typing can be used to rapidly screen five specific LOS types, but more strains are still needed for the validation.

     

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