陈莉萍, 纪蕾, 吴晓芳, 徐德顺. 浙江省湖州市诺如病毒G.17型变异株的全基因组序列分析[J]. 疾病监测, 2020, 35(10): 920-925. DOI: 10.3784/j.issn.1003-9961.2020.10.012
引用本文: 陈莉萍, 纪蕾, 吴晓芳, 徐德顺. 浙江省湖州市诺如病毒G.17型变异株的全基因组序列分析[J]. 疾病监测, 2020, 35(10): 920-925. DOI: 10.3784/j.issn.1003-9961.2020.10.012
Liping Chen, Lei Ji, Xiaofang Wu, Deshun Xu. Whole genome sequence of a norovirus G.17 variant isolated in Huzhou[J]. Disease Surveillance, 2020, 35(10): 920-925. DOI: 10.3784/j.issn.1003-9961.2020.10.012
Citation: Liping Chen, Lei Ji, Xiaofang Wu, Deshun Xu. Whole genome sequence of a norovirus G.17 variant isolated in Huzhou[J]. Disease Surveillance, 2020, 35(10): 920-925. DOI: 10.3784/j.issn.1003-9961.2020.10.012

浙江省湖州市诺如病毒G.17型变异株的全基因组序列分析

Whole genome sequence of a norovirus G.17 variant isolated in Huzhou

  • 摘要:
    目的分析浙江省湖州市检出的诺如病毒GⅡ.17型变异株的基因序列和系统发育与进化关系,为国内诺如病毒的遗传进化研究提供序列参考。
    方法根据GⅡ.17型诺如病毒保守序列,自行设计3对引物,扩增覆盖整个基因组的3个重叠片段,对来自急性胃肠炎病例的一株诺如病毒GⅡ.17型毒株进行全基因序列测定和系统发育和进化分析。
    结果在2015年3月湖州市急性胃肠炎病例标本中分离鉴定出一株诺如病毒株GⅡ.17[P17]变异株(毒株编号:NS15217),获得该病毒基因组全长7556 bp,编码3个开放阅读框(ORF),ORF1长5109 bp(5~5113 nt);ORF2长1623 bp(5094~6716 nt);ORF3长780 bp(6716~7495 nt)。 湖州NS15217毒株在ORF1和ORF2中均属于GⅡ.17型基因型,且与LC037415.1/Hu/GⅡ.17/Kawasaki308和湖州MG692610毒株最接近。 在VP1结构域中有106个插入/缺失/替换的残基,13个(12.3%)在S区,30个(28.3%)位于P1区;大多数替换和插入是位于P2域,有63个氨基酸(59.4%)。
    结论湖州地区2015年分离的诺如病毒GⅡ.17型变异株进化分支属于3b簇。 其全基因组序列将有助于诺如病毒的遗传进化研究以及快速诊断试剂的研制。

     

    Abstract:
    ObjectiveTo analyze the gene sequence, phylogeny and evolution of a norovirus GⅡ. 17 variant isolated in Huzhou, Zhejiang province and provide evidence for the study of genetic evolution of norovirus.
    MethodsAccording to the conserved sequence of GⅡ.17 norovirus, three pairs of primers were designed to amplify three overlapping fragments covering the whole genome for the sequencing, phylogeny and evolution analyses of a norovirus GⅡ. 17 strain isolated from an acute gastroenteritis cases.
    ResultsThe full length of genome of the norovirus GⅡ.17 variant isolated from an acute gastroenteritis in March, 2015 in Huzhou (NS15217) was 7556 bp, encoding 3 ORFs, the length of ORF1 was 5 109 bp (5–5113 nt), the length of ORF2 was 1 623 bp (5094–6716 nt), and the length of ORF3 was 780 bp (6716–7495 nt), respectively. The strain was classified as GⅡ.17 in both ORF1 and ORF2, and was most closely related to the LC037415.1/Hu/GⅡ.17/Kawasaki308 strain and MG 692610 strain isolated in Huzhou. Compared with other strains selected, there were 106 variable residues across the VP1 domain. Among the 106 variable amino acids, 13 (12.3%) were observed in the shell domain and 30 (28.3%) in the P1domain; most of the substitutions and insertions were located in the P2 domain, accounting for 59.4% (63).
    ConclusionThe clade of the norovirus GⅡ.17 variant isolated in Huzhou in 2015 belonged to cluster 3b. The understanding of its whole genome sequence could be helpful for the study of the genetic evolution of the virus and the development of rapid diagnostic reagents and vaccine.

     

/

返回文章
返回