赵文娜, 刘莹莹, 于秋丽, 苏通, 谢赟, 齐顺祥. 2013-2017年河北省卢龙县监测点5岁以下儿童病毒性腹泻流行特征分析[J]. 疾病监测, 2019, 34(2): 132-136. DOI: 10.3784/j.issn.1003-9961.2019.02.010
引用本文: 赵文娜, 刘莹莹, 于秋丽, 苏通, 谢赟, 齐顺祥. 2013-2017年河北省卢龙县监测点5岁以下儿童病毒性腹泻流行特征分析[J]. 疾病监测, 2019, 34(2): 132-136. DOI: 10.3784/j.issn.1003-9961.2019.02.010
Wenna Zhao, Yingying Liu, Qiuli Yu, Tong Su, Yun Xie, Shunxiang Qi. Epidemiological characteristics of viral diarrhea in children under five years old in Lulong county of Hebei, 2013–2017[J]. Disease Surveillance, 2019, 34(2): 132-136. DOI: 10.3784/j.issn.1003-9961.2019.02.010
Citation: Wenna Zhao, Yingying Liu, Qiuli Yu, Tong Su, Yun Xie, Shunxiang Qi. Epidemiological characteristics of viral diarrhea in children under five years old in Lulong county of Hebei, 2013–2017[J]. Disease Surveillance, 2019, 34(2): 132-136. DOI: 10.3784/j.issn.1003-9961.2019.02.010

2013-2017年河北省卢龙县监测点5岁以下儿童病毒性腹泻流行特征分析

Epidemiological characteristics of viral diarrhea in children under five years old in Lulong county of Hebei, 2013–2017

  • 摘要:
    目的分析河北省卢龙县监测点<5岁儿童病毒性腹泻流行特点和病原类型,为病毒性腹泻的防控提供参考依据。
    方法收集2013 — 2017年卢龙县哨点医院<5岁腹泻患儿粪便标本1 451份,采用ELISA法检测轮状病毒(RV)抗原,RV分型鉴定采用巢式反转录–聚合酶链式反应(RT-PCR)法。 人杯状病毒(HuCV)及其分型鉴定和人星状病毒(HAstV)采用RT-PCR法检测。 肠道腺病毒(AV)采用PCR法检测。 采用SPSS 20.0软件对病毒性腹泻结果进行分析。
    结果1 451份粪便标本中,检出至少含有1种病毒核酸968份,总检出率66.71%,混合感染148份,混合感染占全部感染的15.29%。 RV、HuCV、AV、HAstV阳性检出率分别为46.59%、18.88%、8.06%、4.14%。 腹泻病毒总检出率和RV检出率在12 ~ 17月龄最高,分别为76.60%和56.11%,其次为18 ~ 23月龄,且不同月龄间检出率差异均有统计学意义(\chi _\text总^2=52.142,P<0.05;\chi _\rmRV^2=46.218,P<0.05)。 RV阳性检出率高峰出现时间与病毒性腹泻总阳性率一致,均为每年11月开始至次年3月。 RV阳性标本,G分型以G9为优势型(79.29%),P基因型以P[8]型为主(85.50%),G/P组合以G9P[8]为主(75.30%)。 HuCV阳性标本以诺如病毒感染为主。
    结论2013 — 2017年河北省卢龙县病毒性腹泻以RV和HuCV为主要病原体,秋冬季为流行季节。

     

    Abstract:
    ObjectiveTo analyze the epidemiological and etiological characteristics of viral diarrhea in children under five years old in Lulong county, a surveillance area of viral diarrhea, of Hebei province from 2013 to 2017 and provide evidence for the prevention and control of viral diarrhea.
    MethodsA total of 1 451 stool samples were collected from diarrheal children aged <5 years in sentinel hospitals in Hebei province between 2013 and 2017. Rotavirus antigen was detected by using enzyme-linked immunosorbent assay (ELISA) and RV identification and genotyping were conducted with nested RT-PCR. Human caliciviruses (HuCV) and human astrovirus (HAstV) were detected by RT-PCR. Adenovirus was detected by PCR. The data was analyzed by using software SPSS 20.0.
    ResultsAmong 1 451 stool samples, 968 (66.71%) were positive for at least one virus and 148 (15.29%) were positive for more than one virus. The positive rates of RV, HuCV, AV and HAstV were 46.59%, 18.88%, 8.06% and 4.14% respectively. The highest detection rates of diarrhea viruses (76.60%) and RV (56.11%) were recorded in age group 12– 17 months, followed by those in age group 18–23 months. There were significant differences in the positive rates of diarrhea viruses and RV among different age groups (\chi _\rmtotal^2=52.142, P<0.05; \chi _\rmRV^2=46.218, P<0.05). The detection peaks of both diarrhea viruses and RV occurred during November–March. Among the RV strains, G9 was the most common G serotype (79.29%), P[8] was the most common P genotype (85.50%), and most strains belonged to G9P[8] (75.30%). HuCV positive samples were mainly norovirus positive.
    ConclusionRV and HuCV were the main pathogens causing infant diarrhea in Hebei during 2013–2017. Autumn and winter were the high incidence seasons.

     

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