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高艳, 焦阳, 张玉松, 张士尧, 顾琳, 赵剑虹, 王芳, 孙灵利. 2012－2016年北京市朝阳区33株风疹病毒分离株基因特征分析[J]. 疾病监测, 2019, 34(1): 43-47. DOI: 10.3784/j.issn.1003-9961.2019.01.011
引用本文: 高艳, 焦阳, 张玉松, 张士尧, 顾琳, 赵剑虹, 王芳, 孙灵利. 2012－2016年北京市朝阳区33株风疹病毒分离株基因特征分析[J]. 疾病监测, 2019, 34(1): 43-47. DOI: 10.3784/j.issn.1003-9961.2019.01.011
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Yan Gao, Yang Jiao, Yusong Zhang, Shiyao Zhang, Lin Gu, Jianhong Zhao, Fang Wang, Lingli Sun. Genetic characteristic analysis on 33 strains of rubella virus isolated in Chaoyang district of Beijing, 2012–2016[J]. Disease Surveillance, 2019, 34(1): 43-47. DOI: 10.3784/j.issn.1003-9961.2019.01.011
Citation: Yan Gao, Yang Jiao, Yusong Zhang, Shiyao Zhang, Lin Gu, Jianhong Zhao, Fang Wang, Lingli Sun. Genetic characteristic analysis on 33 strains of rubella virus isolated in Chaoyang district of Beijing, 2012–2016[J]. Disease Surveillance, 2019, 34(1): 43-47. DOI: 10.3784/j.issn.1003-9961.2019.01.011
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2012－2016年北京市朝阳区33株风疹病毒分离株基因特征分析

Genetic characteristic analysis on 33 strains of rubella virus isolated in Chaoyang district of Beijing, 2012–2016

    摘要
  • 摘要:
    目的 通过对北京市朝阳区疑似风疹病例咽拭子标本进行细胞培养及基因特征分析，了解其分子流行病学特征。
    方法 使用Vero/SLAM细胞对2012 — 2016年北京市朝阳区暴发和散发病例的风疹咽拭子标本进行病毒分离，应用反转录–聚合酶链式反应扩增风疹病毒E1基因序列，对扩增产物进行核苷酸序列测定。 使用分子生物信息学软件，将获得风疹病毒毒株核酸序列与世界卫生组织推荐风疹病毒13个基因型的32个参考毒株及16株中国部分省市流行株E1基因739个核苷酸序列进行系统发生树的构建及对比分析。
    结果 共分离出33株风疹病毒毒株，其中8株为1E基因型，25株为2B基因型。 1E型风疹流行株分离自2012 — 2013年，2B型风疹流行株分离自2012 — 2016。 2B型流行株组内遗传距离及组间遗传距离均小于1E型流行株的组内遗传距离和组间遗传距离。
    结论 2012 — 2016年北京市朝阳区风疹病毒流行优势株为2B基因型，1E基因型正在逐步被2B基因型代替。 2B基因型风疹流行株核酸序列更稳定，并逐渐成为优势基因型。

     

    Abstract:
    Objective To identify the genotype and the genetic characteristics of rubella virus isolated from throat swabs of suspected rubella cases in Chaoyang district of Beijing during 2012–2016.
    Methods Vero/SLAM cells were used to isolate rubella viruses from throat swabs collected from rubella cases in rubella outbreak and sporadic cases in Chaoyang during this period. The fragments of E1 genes of the isolates were amplified by RT-PCR and then the PCR products were directly sequenced and analyzed. The phylogenetic tree based on the 739 nucleotide sequences of E1 genes were conducted using the rubella virus strains isolated in Chaoyang and 32 WHO reference strain sequences representing 13 genotypes, another phylogenetic tree was also conducted with the 33 rubella virus strains isolated in Chaoyang and 16 rubella virus strains isolated in other provinces in China for the multiple sequence comparison.
    Results A total of 33 strains of rubella virus strains were isolated, which belonged to two different genotypes, i.e. 1E (8 strains) and 2B (25 strains). The genotype 1E strains were detected during 2012–2013, and the genotype 2B strains were detected during 2012–2016. The intra and inter genetic distance of genotype 2B strains were less than those of genotype 1E strains.
    Conclusion Two genotypes of rubella virus (1E and 2B) were detected in Chaoyang during 2012–2016. The nucleotide sequence of genotype 2B strain was more stable and genotype 2B strain has gradually become predominant.

     

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