2007－2017年四川省宋内志贺菌分离株脉冲场凝胶电泳分型及耐药性分析

雷高鹏; 吕虹; 黄玉兰; 黄伟峰; 杨小蓉

doi:10.3784/j.issn.1003-9961.2019.04.014

2007－2017年四川省宋内志贺菌分离株脉冲场凝胶电泳分型及耐药性分析

Drug resistance detection and pulsed-field gel electrophoresis typing of Shigella sonnei isolated in case-based surveillance in Sichuan, 2007–2017

摘要

摘要:
目的了解2007 — 2017年四川省腹泻病例样本宋内志贺菌分离株耐药状况及脉冲场凝胶电泳（PFGE）型别分布。
方法将腹泻病例监测中经过生化和血清学鉴定的30株宋内志贺菌进行PFGE分型分析，采用微量肉汤稀释法测定菌株对14种药物的最低抑菌浓度。
结果宋内志贺菌分离株仅对头孢西丁敏感，多重耐药菌株占总数的96.67%。对环丙沙星、阿奇霉素的耐药率超过10%，对包括头孢噻肟在内的7种常见药物耐药率达到50%以上。分离株可分为16个PFGE型，有2个优势簇占总分离数的80%，其他型别占20%。在优势簇I中的SCSN-1和SCSN-3中各检测到一起疑似暴发事件。
结论2007 — 2017年四川省腹泻病例宋内志贺菌分离株PFGE优势带型明显，且部分带型存在聚集。菌株耐药情况严重，部分菌株对细菌性腹泻常用抗生素环丙沙星、阿奇霉素和三代头孢中的头孢噻肟耐受。

Abstract:
ObjectiveTo understand the drug resistance and pulsed-field gel electrophoresis (PFGE) patterns of Shigella sonnei strains isolated from sporadic diarrhea cases in surveillance in Sichuan province during 2007–2017.
MethodsA total of 30 S. sonnei strains isolated from diarrhea patients were analyzed by PFGE and tested for minimal inhibitory concentration of 14 antibiotics after the serological and biochemical identification.
ResultsThe antibiotic resistance test indicated all the isolates were only sensitive to cefoxitin, while 96.67% of the strains were multi-drug resistant. The resistance rates to azithromycin and ciprofloxacin were more than 10%. The resistance to 7 common antibiotics, including cefotaxime, reached more than 50%. PFGE typing showed that strains had 16 patterns and two predominant clusters which accounted for 80%, and other types accounted for 20%. A suspected outbreak caused by SCSN-1 and SCSN-3 from cluster I was detected respectively.
ConclusionS. sonnei isolated in Sichuan from 2007 to 2017 had two predominant PFGE clusters and partial subtype showed clustering. The strains had high drug resistance, some of which showed resistance to common antibiotics used in the treatment of bacterial diarrhea, such as ciprofloxacin, azithromycin and cefotaxime.

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