刘玉霞, 李红叶, 王炳全, 巩盼龙, 李凡卡. 甲型/乙型副伤寒疫情交叉暴发的病原分析[J]. 疾病监测, 2019, 34(8): 721-724. DOI: 10.3784/j.issn.1003-9961.2019.08.010
引用本文: 刘玉霞, 李红叶, 王炳全, 巩盼龙, 李凡卡. 甲型/乙型副伤寒疫情交叉暴发的病原分析[J]. 疾病监测, 2019, 34(8): 721-724. DOI: 10.3784/j.issn.1003-9961.2019.08.010
Yuxia Liu, Hongye Li, Bingquan Wang, Panlong Gong, Fanka Li. Etiology of paratyphoid A and B outbreaks[J]. Disease Surveillance, 2019, 34(8): 721-724. DOI: 10.3784/j.issn.1003-9961.2019.08.010
Citation: Yuxia Liu, Hongye Li, Bingquan Wang, Panlong Gong, Fanka Li. Etiology of paratyphoid A and B outbreaks[J]. Disease Surveillance, 2019, 34(8): 721-724. DOI: 10.3784/j.issn.1003-9961.2019.08.010

甲型/乙型副伤寒疫情交叉暴发的病原分析

Etiology of paratyphoid A and B outbreaks

  • 摘要:
    目的对2018年新疆生产建设兵团某学校暴发的甲型/乙型副伤寒疫情的病原菌进行分子生物学分析,为暴发疫情的判定及调查工作提供依据。
    方法收集医院病例粪便标本中分离的17株菌株,并进行血清学分型,采用微量肉汤稀释法进行药物敏感性试验,并利用BioNumerics 7.6软件对脉冲场凝胶电泳(PFGE)图谱进行菌株的同源性分析。
    结果17株菌中,12株为甲型副伤寒沙门菌,抗原式为1,2,12∶a∶-;5株为乙型副伤寒沙门菌,抗原式为1,4,12∶b∶1,2。 所有菌株均对14种抗生素敏感。 PFGE图谱聚类分析显示有2种带型,带型相似度为59.4%,相同血清型的菌株具有相同的带型。
    结论PFGE技术在伤寒和副伤寒暴发疫情的分析中发挥着重要作用。

     

    Abstract:
    ObjectiveTo understand the molecular biological characteristics of the pathogens detected in the paratyphoid A and B outbreaks occurred in a school of Xinjiang Production and Construction Corps in 2018, and provide evidence for the identification of the outbreaks.
    MethodsSeventeen strains were collected from patients in the hospital. The serological subtyping of 17 isolated strains was conducted. The antibiotic susceptibility test of the strains was done by using micro-broth dilution method. The pulsed-field gel electrophoresis (PFGE) patterns of the strains were analyzed with software BioNumerics 7.6.
    ResultsAmong the 17 strains, 12 were paratyphoid A strains with antigen 1,2,12∶a∶-, and 5 were paratyphoid B strains with antigen 1,4,12∶b∶1,2. All the strains were sensitive to 14 antibiotics. Cluster analysis of PFGE patterns showed that there were two patterns and the similarity of the two patterns was 59.4%. The same serotype had the same pattern type.
    ConclusionPFGE plays an important role in the investigation of typhoid and paratyphoid outbreaks.

     

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