安徽省北方地区恙虫病血清学诊断和病原体基因序列分析

陈晴晴; 张永根; 刘一梅; 史永林; 龚磊; 吴小珉; 孙永

doi:10.3784/jbjc.202105100257

安徽省北方地区恙虫病血清学诊断和病原体基因序列分析

Serological diagnosis of tsutsugamushi disease and gene sequence analysis of Orientia tsutsugamushi in northern area of Anhui

摘要

摘要:
目的对安徽省北方地区恙虫病临床诊断病例血清学检测分型和病原体基因序列分析，了解恙虫病东方体的基因型流行特征。
方法收集2018年安徽省宿州市、蚌埠市、阜阳市临床诊断为恙虫病患者血液标本92份，采用间接免疫荧光和巢式聚合酶链式反应分别检测病例血清中IgM抗体和血液中恙虫病东方体56 kDa型特异性抗原基因，并对扩增序列进行测序分析。
结果 92份临床诊断为恙虫病患者血液标本中IgM检测阳性61例，血清分型主要为Gilliam；核酸阳性48例，基因分型结果均为Kawasaki相关型。
结论安徽省阜阳市、蚌埠市、宿州市恙虫病东方体基因型主要为Kawasaki相关型，存在暴发风险，需加强恙虫病东方体监测工作。

Abstract:
Objective To analyze the serotype and gene sequence of Orientia tsutsugamushi detected and understand the genotype characteristics of Orientia tsutsugamushi infection in northern area of Anhui province.
Methods A total of 92 blood samples were collected from patients with clinically diagnosed tsutsugamushi disease in Fuyang, Bengbu, Suzhou of Anhui in 2018. The IgM antibodies and partial tsa56 genes of Orientia tsutsugamushi were tested respectively with indirect immunofluorescence assay (IFA) and nested-polymerase chain reaction (nPCR). The sequences of tsa56 genes were analyzed.
Results In 92 serum samples, 61 were IgM positive, Gilliam was the main serotype of Orientia tsutsugamushi detected, and 48 were nucleic acid positive in PCR, the genotypes of all the strains belonged to Kawasaki-related.
Conclusion The genotypes of Orientia tsutsugamushi detected in Fuyang, Bengbu, and Suzhou were Kawasaki-related, it is necessary to strengthen the surveillance for Orientia tsutsugamushi to prevent the outbreak of tsutsugamushi disease in Anhui.

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