新疆古尔图地区长尾黄鼠感染病原体情况调查

李博; 卢珊; 雒涛; 阿布力克木·阿布都热西提; 郝琴; 王信惠; 张琳; 古丽阿依·包开西; 黎唯

doi:10.3784/j.issn.1003-9961.2020.07.015

新疆古尔图地区长尾黄鼠感染病原体情况调查

Infection status of pathogens in Citellus undulatus in Guertu, Xinjiang

摘要

摘要:
目的调查新疆维吾尔自治区（新疆）古尔图地区长尾黄鼠汉赛巴尔通体、伯氏疏螺旋体和嗜吞噬细胞无形体等病原体感染情况，分析该地区自然疫源性疾病流行风险，为制定防控措施提供科学依据。
方法2017年5 — 9月采用一日弓形夹法捕捉查岗果勒、布兰布拉克、白石头等地长尾黄鼠86只，无菌采集鼠体肾脏，试剂盒法提取全基因组DNA；运用实时荧光定量聚合酶链式反应方法检测样本汉赛巴尔通体ssrA基因、伯氏疏螺旋体recA基因和嗜吞噬细胞无形体Msp2基因。
结果共检测86只长尾黄鼠样本，其中汉赛巴尔通体阳性42份，阳性率为48.84%；伯氏疏螺旋体阳性6份，阳性率为6.98%；嗜吞噬细胞无形体阳性3份，阳性率为3.49%。布兰布拉克地区的汉赛巴尔通体感染率高于查岗果勒和白石头地区，白石头地区的伯氏疏螺旋体和嗜吞噬无形体感染率高于其他两地。
结论新疆古尔图地区长尾黄鼠存在汉赛巴尔通体、伯氏疏螺旋体和嗜吞噬细胞无形体感染。

Abstract:
ObjectiveTo investigate the infection status of Bartonella henselae, Borrelia burgdoferi sense lato and Anaplasma phagocytophilum in Citellus undulatus in Guertu of Xinjiang, analyze its transmission risk and provide evidence for control measures.
MethodsA total of 86 Citellus undulatus were captured with 24 hours-bow-shaped clip method in Chagangguole, Bulanlulake and Baishitou of Guertu during May-September, 2017, the kidneys of mouse were removed under aseptic condition, genome DNA was extracted and ssrA of Bartonella henselae, recA of Borrelia burgdoferi sense lato and Msp2 of Anaplasma phagocytophilum were detected by real-time fluorescence quantitative PCR.
ResultsAmong the 86 samples of Citellus undulatus detected, the infection rates of Bartonella henselae, Borrelia burgdoferi sense lato and Anaplasma phagocytophilum were 48.84% (42/86), 6.98% (6/82) and 3.49% (3/86), respectively. The infection rate of Bartonella henselae in Bulanlulake was higher than that in Chagangguole and in Baishitou, the infection rates of Borrelia burgdoferi sense lato and Anaplasma phagocytophilum in Baishitou were higher than those in other two areas.
ConclusionNatural infections of Bartonell henselae, Borrelia burgdoferi sense lato and Anaplasma phagocytophilum were detected in Citellus undulatus in Guertu.

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