Abstract:
Objectives To understand the incidence of diarrhea caused by Campylobacter in Shunyi district of Beijing and explore the detection methods for Campylobacter and analyze the drug resistance characteristics of Campylobacter.
Methods A total of 372 stool samples were collected from patients with diarrhea in two hospitals in Shunyi in 2017 for Campylobacter, Salmonella, Shigella, Diarrheagenic E. coli and Vibrio parahaemolyticus isolation. The filter membrane method was used in the isolation of Campylobacter. The isolated Campylobacter strains were tested for drug sensitivity.
Results Among 372 stool samples, a total of 39 strains of Campylobacter were isolated (10.48%), including 36 strains of C. jejuni (92.31%) and 3 strains of C. coli (7.69%). The positive rates of Salmonella, Shigella, E. coli and V. parahaemolyticus were 6.99%, 0.27%, 9.14%, and 12.63%, respectively. The clinical symptoms of 39 cases of Campylobacter infection were mainly diarrhea (watery stools), abdominal pain, nausea and dehydration. The detection rate of Campylobacter was higher in spring and autumn than in summer and winter, and the peak appeared in October. Campylobacter infection cases were mainly young and middle-aged adults aged 16 to 45 years; there was no significant difference in the detection rate between men and women (P=0.698, χ2=0.150). The infection cases were mainly distributed in students, people engaged in other occupations and in business services. Contaminated foods included vegetables, cereals and aquatic products. Three strains of C. coliwere only sensitive to chloramphenicol. The resistant rates of 36 C. jejuni isolates to quinolones, tetracyclines and chloramphenicol were high, including nalidixic acid (97.22%), ciprofloxacin (97.22%), tetracycline (86.11%), florfenicol (58.33%). Nineteen strains of C. jejuni (52.78%) were multi-drug resistant, and the multi-drug resistance spectrum was mainly quinolones-chloramphenicol-tetracyclines (47.37%, 9/19).
Conclusions The proportion of diarrhea caused by Campylobacter was high in Shunyi. The isolated Campylobacter strains showed serious drug resistance, including multiple drug resistance, to which close attention should be paid.