黄静敏, 柯碧霞, 李柏生, 何冬梅, 刘哲, 李振翠, 邓小玲. 广东省多重耐药沙门菌1,4,[5],12∶i∶-的耐药性及分子流行特征分析[J]. 疾病监测, 2021, 36(5): 501-508. DOI: 10.3784/jbjc.202012020405
引用本文: 黄静敏, 柯碧霞, 李柏生, 何冬梅, 刘哲, 李振翠, 邓小玲. 广东省多重耐药沙门菌1,4,[5],12∶i∶-的耐药性及分子流行特征分析[J]. 疾病监测, 2021, 36(5): 501-508. DOI: 10.3784/jbjc.202012020405
Huang Jingmin, Ke Bixia, Li Bosheng, He Dongmei, Liu Zhe, Li Zhencui, Deng Xiaoling. Molecular epidemiological characteristics and antibiotic resistance of multi-drug resistant Salmonella 1,4,[5],12∶i∶- in Guangdong[J]. Disease Surveillance, 2021, 36(5): 501-508. DOI: 10.3784/jbjc.202012020405
Citation: Huang Jingmin, Ke Bixia, Li Bosheng, He Dongmei, Liu Zhe, Li Zhencui, Deng Xiaoling. Molecular epidemiological characteristics and antibiotic resistance of multi-drug resistant Salmonella 1,4,[5],12∶i∶- in Guangdong[J]. Disease Surveillance, 2021, 36(5): 501-508. DOI: 10.3784/jbjc.202012020405

广东省多重耐药沙门菌1,4,5,12∶i∶-的耐药性及分子流行特征分析

Molecular epidemiological characteristics and antibiotic resistance of multi-drug resistant Salmonella 1,4,5,12∶i∶- in Guangdong

  • 摘要:
      目的  多重耐药沙门菌1,4,5,12∶i∶-的耐药形势日趋严峻,耐药克隆株的广泛传播已成为全球重点关注的公共卫生问题。 了解广东省多重耐药沙门菌1,4,5,12∶i∶-的耐药特征及遗传背景,为该血清型的耐药监测及食源性疾病暴发与防控预警提供证据支持。
      方法  以广东省腹泻病原体监测哨点医院收集的92株沙门菌1,4,5,12∶i∶-为研究对象,采用微量肉汤稀释法测定菌株对27种抗菌药物的最小抑菌浓度,采用全基因组测序技术进行耐药相关分子特征研究。 利用多位点序列分型和全基因组单核苷酸多态性两种方法进行菌株种群结构以及与国际流行克隆株之间的系统发育进化关系分析。
      结果  本研究沙门菌1,4,5,12∶i∶-菌株多重耐药情况严重,对四环素、氨苄西林和磺胺异噁唑呈高水平耐药,对氨基糖苷类、头孢菌素及喹诺酮类呈中度耐药,对喹诺酮类中介敏感率较高。 耐药基因与耐药表型整体相符,携带率高且种类多样,以IncHI2/IncHI2A、IncQ型质粒及插入序列等可移动遗传元件介导的耐药基因传播为本地区沙门菌1,4,5,12∶i∶-的主要耐药机制。 ST34为本地区沙门菌1,4,5,12∶i∶-的优势克隆,与全球的流行株属于同一进化分支,具有较近的遗传进化关系。
      结论  广东地区沙门菌1,4,5,12∶i∶-菌株多重耐药谱广泛,耐药机制较复杂,以获得性耐药为主。 流行菌群具有明确的系统发育分支,以ST34多重耐药克隆为主,与全球流行趋势一致。

     

    Abstract:
      Objective  To study the characteristics of multi-drug resistance of Salmonella 1,4,5,12∶i∶- and its genetic background in Guangdong province, and provide strong support for the drug resistance surveillance of this serotype and the early warning, prevention and control of foodborne disease outbreaks in Guangdong.
      Methods  A total of 92 strains of Salmonella 1,4,5,12∶i∶- collected from the sentinel hospitals for diarrhea pathogen surveillance in Guangdong province were included in the study. Minimal broth dilution method was used to detect the minimum inhibitory concentration of strains to 27 antibacterial agents. Whole-genome sequencing was applied to study the molecular characteristics associated with antibiotic resistance. Multilocus sequence typing (MLST) and whole-genome single nucleotide polymorphisms (wgSNPs) were conducted to analyze population structure of the isolates and evolutionary relationship between the isolates and global circulating strains.
      Results  The multi-drug resistance of Salmonella 1,4,5,12:i:- was serious, with high resistance to tetracycline, ampicillin and sulfisoxazole, moderate resistance to aminoglycosides, cephalosporins, and high sensitivity to quinolones simultaneously. Resistance genes carried by the isolates were various. The drug resistance genes were consistent with overall drug-resistant phenotypes. The transmission of drug resistance genes mediated by mobile genetic elements such as IncHI2/IncHI2A, IncQ-type plasmids and insert sequences was the main mechanism of multi-drug resistance of Salmonella 1,4,5,12∶i∶-. MLST analysis showed that ST34 was the predominant clone of Salmonella 1,4,5,12∶i∶- in Guangdong. Phylogenetic analysis found that the serotype population belonged to the same evolution branch with global circulating strain, which indicated a close genetic evolutionary relationship with international circulating clone.
      Conclusion  The multi-drug resistance patterns of Salmonella 1,4,5,12∶i∶- varied in Guangdong, the drug resistance mechanism was complex, and acquired drug resistance was predominant. The multi-drug resistant Salmonella 1,4,5,12∶i∶- isolates in Guangdong showed a specific phylogenetic lineage. ST34 with a extensive antibiotic resistance pattern was predominant, which was consistent with the global trend.

     

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