杨柳, 徐亚陇, 李金松, 段招军, 马超锋. 20世纪80年代我国142份成年人腹泻粪便标本中常见致腹泻相关病毒筛查[J]. 疾病监测, 2020, 35(3): 218-221. DOI: 10.3784/j.issn.1003-9961.2020.03.009
引用本文: 杨柳, 徐亚陇, 李金松, 段招军, 马超锋. 20世纪80年代我国142份成年人腹泻粪便标本中常见致腹泻相关病毒筛查[J]. 疾病监测, 2020, 35(3): 218-221. DOI: 10.3784/j.issn.1003-9961.2020.03.009
Liu Yang, Yalong Xu, Jinsong Li, Zhaojun Duan, Chaofeng Ma. Detection of common diarrhea-associated viruses in 142 stool samples collected from adult diarrhea patients in 1980s[J]. Disease Surveillance, 2020, 35(3): 218-221. DOI: 10.3784/j.issn.1003-9961.2020.03.009
Citation: Liu Yang, Yalong Xu, Jinsong Li, Zhaojun Duan, Chaofeng Ma. Detection of common diarrhea-associated viruses in 142 stool samples collected from adult diarrhea patients in 1980s[J]. Disease Surveillance, 2020, 35(3): 218-221. DOI: 10.3784/j.issn.1003-9961.2020.03.009

20世纪80年代我国142份成年人腹泻粪便标本中常见致腹泻相关病毒筛查

Detection of common diarrhea-associated viruses in 142 stool samples collected from adult diarrhea patients in 1980s

  • 摘要:
    目的了解我国142份成年人腹泻粪便标本中常见腹泻相关病毒性病原谱。
    方法采用聚合酶链式反应(PCR)检测方法筛查中国疾病预防控制中心病毒病预防控制所毒种库保存的20世纪80年代142份成年人腹泻粪便标本中轮状病毒、诺如病毒、肠道腺病毒和星状病毒等,并测序确定病毒的型别。
    结果120份(84.51%,120/142)粪便标本检出至少1种病毒,其中B组轮状病毒检出率为42.96%(61/142),未检出A组和C组轮状病毒;诺如病毒检出率为0.70%(1/142);肠道腺病毒检出率为4.23%(6/142),其中4型2份,7型2份,40型和41型各1份;星状病毒全部为5型,检出率为34.51%(49/142);猪肠道病毒检出率为1.41%(2/142);柯萨奇病毒检出率为0.70%(1/142);混合感染检出率为24.65%(35/142);脊髓灰质炎病毒未检出。
    结论本批成年人腹泻标本中以B组轮状病毒为主,与星状病毒混合感染率较高,达21.13%(30/142),与标准成年人腹泻B组轮状病毒株核苷酸序列同源性为94.67%~96.52%;其他几种病毒与现有病毒的相似度较高。 从粪便中检出猪肠道病毒,考虑当时的居住环境所致,不能排除标本污染。

     

    Abstract:
    ObjectiveTo understand the common diarrhea-associated viral pathogens in 142 stool samples collected from adult diarrhea patients in 1980s.
    MethodsPolymerase chain reaction was used to detect rotavirus, norovirus, enterovirus and astrovirus in 142 stool samples collected from adult diarrhea patients and stored in virus strain stock of National Institute for Viral Disease Prevention and Control, China CDC since 1980s, and the types of these viruses were determined through sequencing.
    ResultsAt least one virus was detected in 120 stool samples (84.51%, 120/142). The detection rate of rotavirus B was 42.96% (61/142), no rotavirus A and C were detected. The detection rate of norovirus was 0.70% (1/142). The detection rate of intestinal adenovirus was 4.23% (6/142), including two type 4 strains, two type 7 strains, one type 40 strain and one type 41 strain. The detection rate of astroviruses was 34.51% (49/142) and all the strains belonged to type 5. The detection rate of swine enterovirus was 1.41% (2/142). The detection rate of coxsackie virus was 0.70% (1/142). The detection rate of mixed infection was 24.65% (35/142). No poliovirus was detected.
    ConclusionRotavirus B was the main pathogen detected in stool samples collected from adult diarrhea patients in 1980s, and the rate of mixed infection with astrovirus was 21.13% (30/142), and the rotavirus B strains shared 94.67%–96.52% nucleotide sequence homology with standard strain of rotavirus in adult diarrhea patients. Other viruses showed similarities with viruses detected nowadays. Swine enterovirus was detected from the stool samples, which might be explained by sample contamination due to environment condition in 1980s.

     

/

返回文章
返回