2012－2018年山东省济南市A族β溶血性链球菌分子分型特征研究

白爱英; 刘岚铮; 柴政斌; 刘尊玉; 单晓英

doi:10.3784/j.issn.1003-9961.2019.05.006

2012－2018年山东省济南市A族β溶血性链球菌分子分型特征研究

Molecular typing of β-haemolytic group A Streptococcus isolates in Jinan of Shandong, 2012–2018

摘要

摘要:
目的了解济南市A族β溶血性链球菌（GAS)的emm分型特点，为猩红热病原学监测提供数据。
方法收集2012 — 2018年济南市GAS菌株，应用PCR方法扩增emm基因，扩增产物经测序、比对，获得菌株emm基因型。
结果共获得GAS菌株143株，检测到5个emm基因型，其中emm12占55.2%（79/143），emm1占42.0%（60/143），其他emm基因型占2.8%（4/143）。 2015年和2016年仅分到11株菌，均为emm12。患者分离菌株116株，分为4种emm基因型，emm1（46株）和emm12（67株）共占97.4%（113/116），emm22（1株）和emm75（2株）共占2.6%（3/116）。 143株GAS菌株中，菌株数量排在前3位的分别为市中区（85株）、槐荫区（35株）和历城区（9株）。
结论2012 — 2018年济南市GAS菌株优势基因型为emm12和emm1。 2013年和2014年以emm1为主，其余年份以emm12为主。

Abstract:
ObjectiveTo analyze the emm genotypes of β-haemolytic group A Streptococcus (GAS) isolates in Jinan of Shandong province and provide data for etiologic surveillance for scarlet fever.
MethodsGAS isolates were collected in Jinan from 2012 to 2018. The emm gene was amplified by PCR and the emm genotypes were obtained after the PCR products were sequenced and aligned.
ResultsA total of 143 GAS strains were isoalted, belonging to 5 emm genotypes: emm12 (55.2%, 79/143), emm1 (42.0%, 60/143), and other three genotypes (2.8%, 4/143). Only 11 strains were isolated in 2015 and 2016, all belonged to emm12. Four emm genotypes were found in 116 isolates from patients, in which 97.4% were emm1 (46 strains) and emm12 (67 strains), and 2.6% were emm22 (1 strain) and emm75 (2 strains). The first 3 districts where the isolate numbers were high were Shizhong (85 strains), Huaiyin (35 starins) and Licheng (9 strains).
ConclusionThe main emm genotypes of GAS were emm12 and emm1 in Jinan from 2012 to 2018 and emm1 was predominant in 2013 and 2014, while emm12 was predominant in other years.

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