王葳, 陈恺韵, 吴立梦, 任宏, 滕峥. 2017-2020年上海市戊型肝炎病毒基因型特征研究[J]. 疾病监测, 2022, 37(2): 214-219. DOI: 10.3784/jbjc.202106210349
引用本文: 王葳, 陈恺韵, 吴立梦, 任宏, 滕峥. 2017-2020年上海市戊型肝炎病毒基因型特征研究[J]. 疾病监测, 2022, 37(2): 214-219. DOI: 10.3784/jbjc.202106210349
Wang Wei, Chen Kaiyun, Wu Limeng, Ren Hong, Teng Zheng. Genetic characteristics of hepatitis E in Shanghai, 2017–2020[J]. Disease Surveillance, 2022, 37(2): 214-219. DOI: 10.3784/jbjc.202106210349
Citation: Wang Wei, Chen Kaiyun, Wu Limeng, Ren Hong, Teng Zheng. Genetic characteristics of hepatitis E in Shanghai, 2017–2020[J]. Disease Surveillance, 2022, 37(2): 214-219. DOI: 10.3784/jbjc.202106210349

2017-2020年上海市戊型肝炎病毒基因型特征研究

Genetic characteristics of hepatitis E in Shanghai, 2017–2020

  • 摘要:
      目的  研究2017—2020年上海市急性戊型肝炎(戊肝)患者及猪胆汁样本中获得的人源与猪源戊肝病毒(HEV)序列基因型特征。
      方法  对样本进行HEV抗体检测、HEV RNA荧光定量PCR检测、巢式PCR特异性扩增,对获得的HEV核苷酸序列进行深入研究,包括进化分析、基因型及亚型分析、同源性分析等。
      结果  从525份急性戊肝病例血清样本及2400份猪胆汁样本中,共得到191条人源HEV及8条猪源HEV序列:其中98.43%人源HEV为基因4型,包含6种亚型;3条人源HEV与4条猪源HEV为基因3型,包含3种亚型;人源与猪源HEV核苷酸序列的同源性范围为93.36%~100%。
      结论  基因4型HEV在戊肝病例中占绝对优势,同时首次在上海市戊肝病例样本中检测到了基因3型HEV;人源与猪源HEV核苷酸序列高度同源,提示HEV在人与猪之间传播的可能性。

     

    Abstract:
      Objective  To analyze the genetic characteristics of hepatitis E virus (HEV) isolated from human and swine samples collected in Shanghai during 2017–2020.
      Methods  IgM and IgG against HEV were detected with enzyme-linked immunosorbent assay, HEV RNA was detected using qPCR assay, a partial 137-nt sequence of HEV was obtained using nested RT-PCR. Genetic characteristics of HEV isolates were further studied through phylogenetic analysis, including genotype, subtype and nucleotide sequence homology analyses.
      Results  In this study, 191 human HEV isolates and 8 swine HEV isolates were obtained from serum samples of 525 acute hepatitis E patients and bile samples of 2400 pigs respectively. Phylogenetic analysis revealed that 98.43% of human HEV isolates belonged to genotype 4, including subtypes 4a, 4c, 4d, 4e, 4f and 4g, and 3 human isolates and 4 swine isolates belonged to genotype 3, including subtypes 3b, 3c and 3e. The nucleotide sequences of human and swine isolates shared 93.36%–100% homology.
      Conclusion  Genotype 4 HEV was predominant in patients. Human genotype 3 HEV isolates were first reported in Shanghai. Human and swine isolates shared high sequence homology, suggesting the possible zoonotic transmission of HEV between swine and humans.

     

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