2株与霍乱弧菌O139诊断血清发生凝集的麦氏弧菌鉴定

Identification of two Vibrio metschnikovi strains cross-agglutinating with Vibrio cholerae O139

摘要: 目的对2株疑似O139群霍乱弧菌进一步鉴定，以排除或确诊霍乱。方法以试管凝集和交叉吸收实验复核玻片凝集结果，用生化实验和BIOFOSUN 微生物鉴定系统进行生化鉴定，PCR技术检测O139霍乱弧菌特异性脂多糖基因（LPS）。结果玻片凝集阳性，试管凝集1株菌可达原诊断血清效价的1/4，1株菌达1/2，交叉吸收后均证实为非特异性凝集；未检出LPS基因；系统生化鉴定判定为麦氏弧菌。结论2株菌不是O139霍乱弧菌，而是麦氏弧菌。

Abstract: ObjectiveTo identify two strains suspected of Vibrio cholerae O139 to exclude or confirm cholera. MethodsTube agglutination and cross-absorption tests were performed to review the results of slide agglutination. Biochemical experiments featuring BIOFOSUN microbial identification system were conducted, and PCR was applied to detect the Vibrio cholerae O139-specific LPS gene. ResultsThe slide agglutination test was positive. Tube agglutination of one stains demonstrated 1/4 titer of the original serum, and the other 1/2. However, cross-absorption confirmed non-specific agglutination. No Vibrio cholerae O139-specific LPS gene was detected. The biochemical identification system showed that the strains were Vibrio metschnikovi. ConclusionThe two strains were not Vibrio cholerae but Vibrio metschnikovi.