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梁旭东, 杨珊, 马凤琴, 王秉翔. 地高辛标记炭疽杆菌DNA探针的研究[J]. 疾病监测, 1995, 10(12): 357-359. DOI: 10.3784/j.issn.1003-9961.1995.12.357
引用本文: 梁旭东, 杨珊, 马凤琴, 王秉翔. 地高辛标记炭疽杆菌DNA探针的研究[J]. 疾病监测, 1995, 10(12): 357-359. DOI: 10.3784/j.issn.1003-9961.1995.12.357
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Xue Shaohua et al, . A Study on the Etiological Diagnosis and the Factors of Transmission of Hepatitis Type B[J]. Disease Surveillance, 1995, 10(12): 357-359. DOI: 10.3784/j.issn.1003-9961.1995.12.357
Citation: Xue Shaohua et al, . A Study on the Etiological Diagnosis and the Factors of Transmission of Hepatitis Type B[J]. Disease Surveillance, 1995, 10(12): 357-359. DOI: 10.3784/j.issn.1003-9961.1995.12.357
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地高辛标记炭疽杆菌DNA探针的研究

A Study on the Etiological Diagnosis and the Factors of Transmission of Hepatitis Type B

    摘要
  • 摘要: 本文介绍了试用地高辛标记，将炭疽芽孢杆菌的ＰＸＤ１和ＰＸＤ２两质粒上分别扩增到２９０ｂｐ和２８８ｂｐ基因片断制成非放射性标记探针，利用菌落原位杂交方法进行检测，表明两探针的敏感性、特异性均良好，提示该两探针将能为基层工作提供更准确、快速的炭疽杆菌检验技术。

     

    Abstract: Two DNA segments of 290 and 288bp,amplified by PCR from the plasmid Pxo1 and Pxo2 respectively,were labelled with digoxin.IN the in situ hybridization,both of them were sensitive and specific.The results suggested that these two probes could provide more precise and rapid method for the of diagnosis of anthrax surveillance.

     

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