Abstract: From 1992 to 1995,a previous epidemic area and a non-epidemic area both in Guangzhou Haizhu district and Haikang city were selected as the DF surveillance points.Allsuspected DF patients in wholl province were carried out for tested. 1316 mosquitoes divided into 116 groups were tested and no dengue virus was isolated.The sera of 972 healthy persons were tested for dengue antibody (DV-IgG),and 131 sera of them were positive and the titers of most positive sera were 1∶40.DV-IgG was negative in 3 monkey sera.In the same period,1044 sera of suspected dengue fever patients were tested by mosquito cell culture.34 strains of dengue virus were isolated and identified as serotype 2 in 1993 and serotyep 1 in 1995.198 sera samples of suspected dengue cases were tested for DVP-IgG and 55 specimens were positive,2of them were the paired sera from the same patient in 1993. The types of them could not identified due to the severe cross-reaction.