Abstract: The etiological and serological surveillance for acute hemorrhagical conjunctivitis(AHC) were carried out in 19881997. Using HeLa cell tissue culture, CoxA24 Variants (CA24V) were isolated from 25 of 37 samples. Paired serum samples from 8 of AHC patients were examined for neutralizing antibody. There were >4 fold rise in antibody tites. After two years, the antibody level of patients who had suffered from AHC became lower. The results indicated that CA24V was the etiological agent in the epidemic of AHC in beijing area.So our experiment IIF was a good way for rapid diagnosis.It was also observed that the AHC antibody level was lower in normal population,so,AHC maybe happened once again in Beijing.