Abstract:
Objective. To set up a simple, practical method for detecting minimun polio virus in large volume water. Method. Adding L20B cells into the large volume water containing minimum polio virus. Collecting the cells after they were fully reacted with viruse, then isolated, the method of NaCl-AlCl 3 sedimentation as control. Result. If the amount of viruse was 100, 10TCD 50 per 1000ml water, the viruse could be detected. When the amount was 1~2TCD 50 , the viurse in 13 of 14 samples could be detected. While the viruse of 100TCD50 per 1000ml water only in 1 of 4 samples could be detected with the method of NaCl AlCl3 sedimentation.