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狄飚, 伍颂民, 周秀珍, 徐建敏. 微量细胞培养法在分离流感病毒中的应用[J]. 疾病监测, 2001, 16(1): 5-7. DOI: 10.3784/j.issn.1003-9961.2001.1.5
引用本文: 狄飚, 伍颂民, 周秀珍, 徐建敏. 微量细胞培养法在分离流感病毒中的应用[J]. 疾病监测, 2001, 16(1): 5-7. DOI: 10.3784/j.issn.1003-9961.2001.1.5
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DI Biao. et al, . ISOLATION OF INFLUENZA VIRUSE BY USING MICROPLATE METHOD[J]. Disease Surveillance, 2001, 16(1): 5-7. DOI: 10.3784/j.issn.1003-9961.2001.1.5
Citation: DI Biao. et al, . ISOLATION OF INFLUENZA VIRUSE BY USING MICROPLATE METHOD[J]. Disease Surveillance, 2001, 16(1): 5-7. DOI: 10.3784/j.issn.1003-9961.2001.1.5
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微量细胞培养法在分离流感病毒中的应用

ISOLATION OF INFLUENZA VIRUSE BY USING MICROPLATE METHOD

    摘要
  • 摘要: 为尝试流行性感冒病毒分离的新方法 ,应用微量细胞培养法与试管培养法同时对 6.18份患者的漱口液标本进行流感病毒分离 ,并比较两种方法的差别。结果表明 ,微量细胞培养法共分离出10.4株病毒 ,分离率16.83% ,其中甲1 型 (H1N1) 2株 ,甲3 型 (H3N2 ) 90株 ,乙型 12株。试管培养法共分离到病毒 93株 ,分离率15.05 %。因此 ,微量细胞法在分离大批量标本时 ,其操作简便 ,省时省力 ,准确可靠的优点尤其突出。

     

    Abstract: The authors conducted a comparative study on 618 throat wash specimens for isolating influenza virus using the microplate method and the tube method. Of them 104 strains for virus (positive rate 16.83%) were isolated with microplate method, including 2 strains of influenza A(1N1), 90 strains of influenza A(H 3M2) and influenza B(12 strains), while 93 strains of virus were isolated with the tube method (positive rate 15.05%). But the microplate method was more simple, convenient and time-saving than the tube method.

     

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