贵州省2000年甲型副伤寒沙门菌分离株16SrRNA基因型分析

Analysis on 16s rRNA Ribotype of Salmonella paratyphi A Strains Isolated from Guizhou Province,2000

摘要: 近年来贵州省出现甲型副伤寒的流行。为探讨贵州省不同地区甲型副伤寒流行菌株的分子流行病学特征以及菌株间的相关关系 ,对贵州省 6个地 (市 ) 2 0 0 0年部分甲型副伤寒沙门菌分离株进行染色体DNA基因限制性酶切 16srRNA探针杂交图谱分析。PstⅠ酶切后进行的杂交显示 6 4株均为一个基因型 ,然后进一步采用BglⅠ、SalⅠ两个内切酶对其中部分菌株进行酶切杂交分析 ,核糖体杂交谱上仍没有差异 ,分析认为同一个克隆群的菌株广泛传播是造成贵州省甲型副伤寒流行的主要原因.

Abstract: The prevalence caused by Salmonella paratyphi A was occurred recently in Guizhou province. To analyze the molecular epidemiological characteristics of the strains isolated from different districts in Guizhou, 64 strains in year 2000 were analyzed by hybridization chromosomal DNA restriction fragments length polymorphism with 16s Rrna gene probe, I.e. Ribotyping. All strains were the same hybridization pattern when the DNA was digested with PstI, although the DNA of some strains was further digested and analyzed with BglI,respectively.It was suggested that the widely spread of the same clone of strains might be the major cause in the prevalence of S.paratyphi A in Guizhou.