重组核衣壳蛋白用于肾综合征出血热病人IgG抗体检测

Detection of Serological serotypes of HantavirusInfections by Recombinant Nucleocapsid Proteinsand Discovery of DOB Serotype in China

摘要: 目的检测肾综合征出血热病人的IgG抗体及发现在我国可能存在的新的血清型。方法用五种血清型汉坦病毒核衣壳重组蛋白作抗原建立间接ELISA法,检测HFRS病人血清中特异性IgG。结果检测了91份病人血清,发现我国山东、北京地区的汉坦病毒感染以HTN血清型群为主(56.1％);首次检测到2份疑似DOB型病毒感染血清。结论我国有可能存在DOB病毒血清型。

Abstract: Objective To detect the IgG of HFRS patient sera and find new serotypein in China. Methods The indirect ELISA was established based on 5 different recombinant NP antigens. The specific IgG of HFRS patient sera were determined.Results Out of 91 sera,57 was positive of specific antibody (62.6%);The majority of the sera (32/ 57)were belong to HTNgroup. ( sera were multiple - type (React to 5 different antigens,but no significant difference was found between individual A450). And 2 sera were determined as DOB serotype.Conclusions The results of the sero-logical evidence indicate that DOB serotype might exist in China.