Abstract: Objective The situation of domestic animals, fowls and meat wares contaminated with E.coli O157∶H7 was under investigation. Methods General cultivation method and immunomagnetic separation were used, during the E.coli O157∶H7 infection period in Shanghai, to ivestigate feces of domestic animals and fowls, and meat products, collected in surveillance spots and supermarkets in the year 2000 and 2001 respectively. Result An overall positive rate with 3.90% E.coli O157∶H7 was obtained. Meat has the highest positive rate,4.87%.And the positive rate was higher in 2001 than in 2000.Form the result of 16 antibiotic resistant surveillance,the rate of bacterial strains from different sources resistant to 13 antibiotics differs.Conclusion The contamination with E.coil O157:H7 existed among domestic animals,fowls and meat products in Shanghai,and meat products have a higher positive rate than domestic animals,fowls.The immunomagnetic separation has a higher positive rate than general cultivation method.