2002年广州地区暴发的登革热流行特点分析
Epidemic Characteristics of Dengue Fever in Guangzhou Prefecture 2002
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摘要: 目的 了解2002年广州地区暴发登革热(DF)的流行特点,为其诊断、预防和治疗提供依据。方法 采用间接酶联免疫(ELISA)法、免疫斑点法、免疫层析法,对3 716例发热待查、疑似患者的血清特异性抗体IgM、IgG进行检测。用C6/36细胞对DF病例176份标本进行病毒分离,以间接免疫荧光(McAb-IFA)、RT-PCR方法鉴定。结果 本次广州地区暴发流行的登革热血清学确诊病人为886人。流行开始于5月份,8、9、10 月份达高峰期,11月有明显下降。发病以青壮年为主。在3 716例发热待查、疑似患者中,血清IgG、IgM抗体阳 性886例,阻性率27.84％。病毒分离阳性46份,阳性率26.1％。经单克隆抗体间接免疫荧光(McAb-IFA)、 RT-PCR检测鉴定为I型登革热病毒。结论 在疾控工作中,应加强Ⅰ型登革热病毒的监测工作。Abstract: Objective To explore the epidemic characteristics of dengue fever in Guangzhou prefecture in 2002. Methods Serum samples from 3 716 patients with suspected dengue fever were assayed by ELISA Immunoblot and Immunochromatography to detect the antibody of IgM and IgG. 176 serum samples of dengue fever were made to isolate virus with C6/36 cell line and the positive serum were identified by McAb ?IFA and RT-PCR. Results The serum antibody of IgG, IgM from 886/3 716 (23. 84%) patients were positive. The dengue virus were isolated from 46 serum and the isolation positive rate was 26.1%.This dengue outbreak lasted a longer period of starting on May,climbing to the peak on September and falling ob-viously on November. Conclusions The pathogen of dengue fever in Guangzhou 2002 was the dengue virus I. We should strengthen the surveillance work of dengue virus I.
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