Abstract: Objective To explore the HBV mark’s changing tendence in Rizhao City.Method To adopt the simple random sampling method to investigate 405 persons of different age in Donggang district; and to collect 2 ml of vein blood to examine HBsAg and anti-HBs with ELA method.Results The average rate of HBsAg carrying was 2.96%,and the highest rate of HBsAg crrying was in the group of 20-40 years old(5.46%).There were no significant different among other groups.Meanwhile,the average positive rate of anti-HBs was 60.99%,the highest positive rate was in the group of <2 years old (81.54%),and the lowest positive rate was in the group of 8 years old. There was a statistical significant different between them. In people who had HBV vaccine immunity experience,the positive rate of anti- HBs was 62.50%,and in those who hadn't HBV vaccine immunity experience was 36.07%,Conclusions The inoculability of HBV vaccine was proved to be efficacious against HBV,but positive rate of anti-HBs declined rapidly after 4 years of HBV vaccine inoculability. In order to improve the positive rate of anti-Hbe, it was necessary to augment immunity to neonates who were negative of anti-HBs after 5 years of the whole course of HBV vaccine in-ocubalityl Otherwise,it wasn't neccssary to apply augmenting immunity after 10 years.