景怀琦, 邱海燕, 李继耀, 肖玉春, 崔志刚, 顾玲, 汪华, 黄晓蓉, 阚飙, 徐建国. 小肠结肠炎耶尔森菌O∶3血清型特异性单克隆抗体制备[J]. 疾病监测, 2004, 19(7): 247-250. DOI: 10.3784/j.issn.1003-9961.2004.7.247
引用本文: 景怀琦, 邱海燕, 李继耀, 肖玉春, 崔志刚, 顾玲, 汪华, 黄晓蓉, 阚飙, 徐建国. 小肠结肠炎耶尔森菌O∶3血清型特异性单克隆抗体制备[J]. 疾病监测, 2004, 19(7): 247-250. DOI: 10.3784/j.issn.1003-9961.2004.7.247
JING Huaiqi. et al, . Preparation of specific mAb of Yersinia Enterocolitica Serotype O∶3[J]. Disease Surveillance, 2004, 19(7): 247-250. DOI: 10.3784/j.issn.1003-9961.2004.7.247
Citation: JING Huaiqi. et al, . Preparation of specific mAb of Yersinia Enterocolitica Serotype O∶3[J]. Disease Surveillance, 2004, 19(7): 247-250. DOI: 10.3784/j.issn.1003-9961.2004.7.247

小肠结肠炎耶尔森菌O∶3血清型特异性单克隆抗体制备

Preparation of specific mAb of Yersinia Enterocolitica Serotype O∶3

  • 摘要: 目的 制备出小肠结肠炎耶尔森菌血清O∶3型特异的单克隆抗体。方法 用常规的骨髓杂交瘤融合技术。结果 筛选到了一株针对于小肠结肠炎耶尔森菌O∶3血清型特异性的克隆株 (编号 :Y6H8) ,且证实分泌的抗体属于针对于脂多糖免疫球蛋白IgG ,可用于玻片凝集法鉴定 0∶3血清型小肠结肠炎耶尔森菌。 结论 通过本实验证实小肠结肠炎耶尔森菌血清O∶3型菌株脂多糖存在其特异性成分 ,可用于该菌株的分型鉴定.

     

    Abstract: Objective Preparation of specific mAb of Yersinia enterocolitica serotype O:3. Method Regular marrow hybridoma fusion technology was used in this study. Result One specific mAb of Yersinia enterocolitica serotype O:3 was obtained and was confirmed to be the specific monoantibody to lipopolysaccharide IgG. It could be used to identify Yersinia enterocolitica serotype O:3. Conclusion The specific motif presented in the lipopolysaccharide of Yersinia enterocolitica serotype O:3 could be used to type and identify the strains.

     

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