荧光定量PCR法与ELISA法检测乙肝病毒感染相关性的研究
A Study of the Correlation Between Fluorescent Quantitative PCR and ELISA in Detection of HBV Infection
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摘要: 目的 采用荧光定量PCR法与ELISA法检测乙肝病毒标志物 ,探讨两种检测方法的相关性。方法 对 95 2份深圳服务行业从业人员体检乙肝表面抗原阳性的血清 ,应用HBV荧光定量PCR法和ELISA法检测并对结果进行分析。结果 荧光定量PCR检测不同乙肝标志物分组阳性率存在一定的差异 ;E抗原阳性的HBVDNA阳性对数均值为 6 . 4 9± 1 .2 9,表面抗原阳性而E抗原阴性的HBVDNA阳性对数均值为 4. 4 6± 1. 2 9,两者间差异具有显著性 (P <0 . 0 1) ,而不同性别和籍贯间无明显差异。结论 两种方法检测乙肝标志物有密切的相关性 ;对从业人员用ELISA法检测乙肝表面抗原阳性者应进一步进行荧光定量PCR检测。Abstract: Objective Fbrescent quantitative PCR and ELISA were used for the detaction of Hepatitis B virus markers and correlation between the two melhods was evaluated Methods 952 serum sam- ples from service industries staff with HBV surface antigen(HBsAg)positive in Shenzhen were detectedand analyzed by HHV fluorescent quantitative PCR and ELISA respectively Results Definite differ-ences exited among groups of different HBV markers by fluorescent quantitative PCR.The positive logarithmic average value of HBV DNA in patients with positive E antigen(HBeAg)was 6.49±1.29，while the logarithmic average value of positive HBV DNA in patients with negative HB but positive HBsAg was 4.46±1.29. There was a significam difference between them(P<0.01)，but no apparent differ-ences were found as far as sexes and native places were concemed Conelusion A close correlation exists between these two methods in the detection of HBV markers；HBsAg-positive patients detected by ELISA should be further tested by fluorescent quantitative PCR.
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