Abstract: Objective The present study was conducted to observe the laboratory survival status of V. Cholera in the water quality of Minjiang River．Methods The water specimens were col-lected from three different water area in Minjiang River of Fuzhou，which was divided into smaller vessels and sterilized，adding an amount of V. Cholera 0139 and V.cholerae serovar．Ogawa to wa- ter specimens，respectively，stonng at the room temperature，being observed and identified termly．The virulence gene of survival strain in water was detected．Results The lab survival time of V.Cholera in the water at the mouth of Guanto and Tingjiang were longer than that at Hongshan-qiao in Minjiang River．The lab survival time of V．Cholera in summer and autumn were longer—than that in winter．The lab survival time of V.cholera 0139 strain was longer than that of V cholerae serovar．Ogawa．The virulence of two strains was all detected after surviving in water specimens．Conclusions The survival time of V．Cholera was associated with different water quali-ty，seasonality and strain type．The virulence remained after surviving in water．