外周血采集方法检测乙型肝炎表面抗原的探讨

Study on the Detection of Surface Antigens of Hepatitis B by Sampling Peripheral Blood

摘要: 目的　探讨外周血采样方法检测乙型肝炎表面抗原的可行性。方法　抽取体检者静脉血,同时用加有EDTA - 2K的带盖离心管采集外周血,分别用两种血检测乙型肝炎表面抗原。结果　检测5 32份样本,外周血检测乙型肝炎表面抗原阳性5 0例,阳性率为9.39% ;静脉血检测乙型肝炎表面抗原阳性5 2例,阳性率为9.77%。两种方法检测结果的差异无显着性。结论　可以用外周血采样法代替静脉血采样法检测乙型肝炎表面抗原。

Abstract: Objective This study was intended to probe the feasibility of applying peripheral blood sampling to the detection of surface antigens of hepatitis B．Methods Venous blood of examinees was drawn along with sampling of peripheral blood by EDTA-2K-additive lidded centrifuge tubes．Both of which were used to determine surface antigens of hepatitis B．Results 532 specimens were tested．50 positive cases were detected by peripheral blood sampling，with a rate of 9.39％，while 52 were identi-fled by venous blood detection．With a rate of 9.77％．The results of both methods had no significant difference．Conclusion Venous blood can be replaced by peripheral blood to determine surface antigens of hepatitis B．