90例非轮状病毒腹泻患儿粪便中诺瓦克样病毒的检测

Determination of Norwalk-like Viruses in Feces of Children with Nonrotavirus Diarrhea

摘要: 目的了解90份婴幼儿腹泻病人轮状病毒分子生物学方法检测阴性的粪便标本中诺瓦克样病毒感染状况。方法应用酶联免疫吸附实验方法(ELISA)进行诺瓦克样病毒抗原检测。结果90份粪便标本中诺瓦克样病毒I型(G1)和Ⅱ型(G2)阳性分别检出6例和19例,阳性率分别为6.67%和21.11%,两者差异有显著性。结论诺瓦克样病毒感染可能是武汉地区婴幼儿腹泻的重要病因之一,其中基因Ⅱ型为主要流行株。

Abstract: Objective This study was made to understand the infection status of Norwalk-like virus-es in 90 rotavirus-negative stool samples tested by molecular biology from infants and pre-school children who Suffered from diarrhea．Methods Enzyme-linked immunosorbent assay (ELISA) was adopted to determine the antigen of Norwalk-like viruses．Results 6 cases of type I(G1)and 19 cas-es of type II (G2) Norwalk-like virus were detected in 90 stool samples，with a positive rate of 6.67％ and 21.11％，respectively，indicating significant difference between them．Conclusion The in-feetion of Norwalk-like viruses might be one of the most important factors that lead to diarrhea among infants and pre-school children in Wuhan city，typeⅡ Norwalk一like virus iS the main epidemic strain．